Meiotic DNA Synthesis during Mouse Spermatogenesis

The incorporation of radioactivity into various cells in the sequence of spermatogenesis was measured by preparing highly purified spermatozoan nuclei from the cauda epididymidis of mice at daily intervals after injection of [3H]thymidine. The stages of differentiation of these sperm at the time of...

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Bibliographic Details
Published in:The Journal of cell biology 1975-01, Vol.64 (1), p.211-222
Main Authors: Meistrich, Marvin L., Reid, Betty O., Barcellona, Wayne J.
Format: Article
Language:English
Subjects:
Animals
Arginine - metabolism
Cell Differentiation
Cell nucleus
Cell Nucleus - metabolism
Cells
CHO cells
Deoxyribonucleases
Dithiothreitol
DNA
DNA - biosynthesis
Epididymis
Germ cells
Kinetics
Male
Meiosis
Mice
Mice, Inbred Strains
Microscopy, Electron
Nucleoproteins - biosynthesis
Polyethylene Glycols
Pronase
Prophase
Radioactive decay
Sertoli cells
Sodium Chloride
Sodium Dodecyl Sulfate
Spermatogenesis
Spermatozoa
Spermatozoa - metabolism
Spermatozoa - ultrastructure
Thymidine - metabolism
Time Factors
Tritium
Trypsin
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Summary:The incorporation of radioactivity into various cells in the sequence of spermatogenesis was measured by preparing highly purified spermatozoan nuclei from the cauda epididymidis of mice at daily intervals after injection of [3H]thymidine. The stages of differentiation of these sperm at the time of thymidine administration were calculated from the kinetics of spermatogenesis. The procedure for purification of sperm nuclei included sonication, mechanical shearing, and treatment with trypsin, DNase, Triton X-100, 2 M NaCl, and sodium dodecyl sulfate. DNA was isolated from these nuclei by treatment with dithiothreitol and pronase, followed by phenol extraction and ethanol precipitation. The levels of radioactivity in the epididymal sperm head preparations were low (
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.64.1.211