Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas [reinhardtii, algae]

Cells of Chlamydomonas reinhardtii were pulse-labeled in vivo in the presence of inhibitors of cytoplasmic (anisomycin) or chloroplast (lincomycin) protein synthesis to ascertain the sites of synthesis of chloroplast ribosomal proteins. Fluorographs of the labeled proteins, resolved on two-dimension...

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Bibliographic Details
Published in:The Journal of cell biology 1983-05, Vol.96 (5), p.1451-1463
Main Authors: Schmidt, Robert J., Richardson, Carol B., Gillham, Nicholas W., Boynton, John E.
Format: Article
Language:English
Subjects:
Anisomycin - pharmacology
Cells
Chlamydomonas - metabolism
Chloroplasts
Chloroplasts - drug effects
Chloroplasts - metabolism
Cytoplasm
Electrophoresis, Polyacrylamide Gel
Gels
Genetic mutation
Lincomycin - pharmacology
Macromolecular Substances
Molecular Weight
Plant cells
Protein synthesis
Ribosomal proteins
Ribosomal Proteins - biosynthesis
Ribosomes
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Summary:Cells of Chlamydomonas reinhardtii were pulse-labeled in vivo in the presence of inhibitors of cytoplasmic (anisomycin) or chloroplast (lincomycin) protein synthesis to ascertain the sites of synthesis of chloroplast ribosomal proteins. Fluorographs of the labeled proteins, resolved on two-dimensional (2-D) charge/SDS and one-dimensional (1-D) SDS-urea gradient gels, demonstrated that five to six of the large subunit proteins are products of chloroplast protein synthesis while 26 to 27 of the large subunit proteins are synthesized on cytoplasmic ribosomes. Similarly, 14 of 31 small subunit proteins are products of chloroplast protein synthesis, while the remainder are synthesized in the cytoplasm. The 20 ribosomal proteins shown to be made in the chloroplast of Chlamydomonas more than double the number of proteins known to be synthesized in the chloroplast of this alga.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.96.5.1451