In vitro Induction of Cartilage-Specific Macromolecules by a Bone Extract

An in vitro system has been developed to study the onset of chondrogenesis. Embryonic rat muscle mesenchymal cells, when treated in suspension culture with an extract of bovine bone matrix, synthesized cartilage-specific proteoglycan and type II collagen. The synthesis of these two macromolecules wa...

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Bibliographic Details
Published in:The Journal of cell biology 1983-12, Vol.97 (6), p.1950-1953
Main Authors: Seyedin, Saeid M., Thompson, Andrea Y., Rosen, David M., Piez, Karl A.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Bone and Bones - physiology
Bones
Cartilage
Cartilage - drug effects
Cartilage - metabolism
Cell culture techniques
Cells, Cultured
Chemical suspensions
Chondrocytes
Collagen - biosynthesis
Collagens
Cultured cells
Enzyme-Linked Immunosorbent Assay
Female
Fetus
Fundamental and applied biological sciences. Psychology
Gels
Glycoproteins
Macromolecules
Muscles - embryology
Muscles - physiology
Pregnancy
Proteins
Proteoglycans
Proteoglycans - biosynthesis
Rapid Communications
Rats
Rats, Inbred Strains
Tissue Extracts - pharmacology
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Summary:An in vitro system has been developed to study the onset of chondrogenesis. Embryonic rat muscle mesenchymal cells, when treated in suspension culture with an extract of bovine bone matrix, synthesized cartilage-specific proteoglycan and type II collagen. The synthesis of these two macromolecules was assayed by the enzyme-linked immunosorbent assay inhibition technique. Further evidence of chondrogenesis was demonstrated by morphological changes of treated cells when cultured in firm agarose and stained for metachromatic matrix. Even with crude bone matrix extracts, the assay was sensitive at the microgram level and significant differences in cartilage macromolecules compared with controls were observed in 2-3 d. In vivo the same extract induced first cartilage and then bone.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.97.6.1950