Recycling of Platelet Phosphorylation and Cytoskeletal Assembly

• Published in: The Journal of cell biology 1984-01, Vol.98 (1), p.8-15
• Main Authors: Cox, A. Chadwick, Carroll, Roger C., White, James G., Gundu H. R. Rao
• Format: Article
• Language: English
• Subjects: Actins; Actins - metabolism; Aggregation; Arachidonic Acid; Arachidonic Acids - pharmacology; Biochemistry; Biological and medical sciences; Blood coagulation. Blood cells; Blood Platelets - ultrastructure; Carrier Proteins - metabolism; Cytoskeleton - physiology; Epoprostenol - pharmacology; Fibrinogen - metabolism; Fundamental and applied biological sciences. Psychology; Gels; Gelsolin; Humans; Microfilament Proteins; Microscopy, Electron; Molecular and cellular biology; Myosins - metabolism; Phosphoproteins - blood; Phosphorylation; Physiological regulation; Platelet; Platelet activation; Platelet Aggregation; Platelets; Protein Binding; Radioactive decay
• ISSN: 0021-9525; 1540-8140
• DOI: 10.1083/jcb.98.1.8

The shape change and aggregation of washed platelets induced by 10 μM arachidonic acid (AA) can be reversed by 20 ng/ml prostacyclin ( PGI2, but these platelets can be reactivated by treatment with 30 μM epinephrine and subsequent addition of 10 μM AA mixture. These events may be modulated by cAMP since 2 mM dibutyryl cAMP also reversed activation without reactivation by epinephrine and AA. We examined protein phosphorylation and formation of cytoskeletal cores resistant to 1% Triton X-100 extraction of these platelets and correlated these processes with aggregation, fibrinogen binding, and changes in ultrastructure. Unactivated platelet cores contained