To Grow Mouse Mammary Epithelial Cells in Culture

Normal mouse mammary epithelial cells from Balb/c mice were successfully cultivated on tissue culture plastic with lethally irradiated LA7 feeder cells. The feeder cells also promoted colony formation from single mouse mammary cells, and the fraction of cells that formed colonies was proportional to...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 1984-03, Vol.98 (3), p.1026-1032
Main Authors: Ehmann, U. K., Peterson, W. D., Misfeldt, D. S.
Format: Article
Language:English
Subjects:
Animal cells
Animals
Biological and medical sciences
Biotechnology
Cell culture techniques
Cell cultures. Hybridization. Fusion
Cell Division
Cell growth
Cell lines
Cells
Cells, Cultured
Cultured cells
Epithelial Cells
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Feeder cells
Female
Fibroblasts
Flasks
Fundamental and applied biological sciences. Psychology
Mammary Glands, Animal - cytology
Mammary Glands, Animal - ultrastructure
Methods. Procedures. Technologies
Mice
Microscopy, Electron
Molecular and cellular biology
Rats
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Normal mouse mammary epithelial cells from Balb/c mice were successfully cultivated on tissue culture plastic with lethally irradiated LA7 feeder cells. The feeder cells also promoted colony formation from single mouse mammary cells, and the fraction of cells that formed colonies was proportional to the density of feeder cells. The mouse mammary cells could be passaged at least 8-12 times as long as new feeder cells were added at each passage. The cells now in culture have doubled in number at least 30 times, but the in vitro lifespan is not yet known. The cultures of mouse cells maintained by this technique never became overgrown with fibroblasts and numerous domes formed in the cultures.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.98.3.1026