Basal Lamina Formation by Cultured Microvascular Endothelial Cells

The production of a basal lamina by microvascular endothelial cells (MEC) cultured on various substrata was examined. MEC were isolated from human dermis and plated on plastic dishes coated with fibronectin, or cell-free extracellular matrices elaborated by fibroblasts, smooth muscle cells, corneal...

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Bibliographic Details
Published in:The Journal of cell biology 1984-08, Vol.99 (2), p.692-698
Main Authors: Kramer, R. H., Bensch, K. G., Davison, P. M., Karasek, M. A.
Format: Article
Language:English
Subjects:
Animals
Aorta - physiology
Basement membrane
Biological and medical sciences
Cell coat. Cell surface
Cell culture techniques
Cell lines
Cell structures and functions
Cells
Cells, Cultured
Collagens
Cultured cells
Endothelial cells
Endothelium - physiology
Endothelium - ultrastructure
Epidermal cells
Extracellular matrix
Fibroblasts - physiology
Fundamental and applied biological sciences. Psychology
Humans
Infant, Newborn
Male
Microcirculation - physiology
Microcirculation - ultrastructure
Microscopy, Electron
Molecular and cellular biology
Muscle, Smooth, Vascular - physiology
Muscle, Smooth, Vascular - ultrastructure
Rats
Skin Physiological Phenomena
Ungulates
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Summary:The production of a basal lamina by microvascular endothelial cells (MEC) cultured on various substrata was examined. MEC were isolated from human dermis and plated on plastic dishes coated with fibronectin, or cell-free extracellular matrices elaborated by fibroblasts, smooth muscle cells, corneal endothelial cells, or PF HR9 endodermal cells. Examination of cultures by electron microscopy at selected intervals after plating revealed that on most substrates the MEC produced an extracellular matrix at the basal surface that was discontinuous, multilayered, and polymorphous. Immunocytochemical studies demonstrated that the MEC synthesize and deposit both type IV collagen and laminin into the subendothelial matrix. When cultured on matrices produced by the PF HR9 endodermal cells MEC deposit a subendothelial matrix that was present as a uniform sheet which usually exhibited lamina raraand lamina densa-like regions. The results indicate that under the appropriate conditions, human MEC elaborate a basal lamina-like matrix that is ultrastructurally similar to basal lamina formed in vivo, which suggests that this experimental system may be a useful model for studies of basal lamina formation and metabolism.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.99.2.692