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ATP-Induced Gelation-Contraction of Microtubules Assembled in vitro

We report here an ATP-dependent formation and contraction, or syneresis, of a microtubule gel using microtubule proteins prepared from calf brains. Gel contraction is typically observable 15-30 min after ATP addition to microtubules assembled to steady state, and is complete after ∼60 min, at which...

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Bibliographic Details
Published in:The Journal of cell biology 1984-10, Vol.99 (4), p.1527-1533
Main Authors: Weisenberg, Richard C., Cianci, Christopher
Format: Article
Language:English
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Summary:We report here an ATP-dependent formation and contraction, or syneresis, of a microtubule gel using microtubule proteins prepared from calf brains. Gel contraction is typically observable 15-30 min after ATP addition to microtubules assembled to steady state, and is complete after ∼60 min, at which time the gel volume is reduced by as much as 75%. In contracted gels, microtubule bundles and aster-like structures are observable. Gelation-contraction requires only microtubule proteins present after purification by three cycles of assembly and disassembly.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.99.4.1527