The Pupal Cuticle of Drosophila: Differential Ultrastructural Immunolocalization of Cuticle Proteins

Precise ultrastructural localization of Drosophila melanogaster pupal cuticle proteins (PCPs) was achieved by the immunogold labeling of frozen thin sections. PCPs were found in lamellate cuticle and intracellular vesicles but, curiously, were absent from the assembly zone of the cuticle. Antibodies...

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Bibliographic Details
Published in:The Journal of cell biology 1986-01, Vol.102 (1), p.306-311
Main Authors: Wolfgang, William J., Fristrom, Dianne, Fristrom, James W.
Format: Article
Language:English
Subjects:
Animal cuticle
Animals
Antibodies
Antiserum
Biochemistry. Physiology. Immunology
Biological and medical sciences
cuticle
Drosophila
Drosophila melanogaster
Drosophila melanogaster - ultrastructure
Drosophilidae
Epidermis
Fundamental and applied biological sciences. Psychology
Insect cuticle
Insect Hormones - metabolism
Insect larvae
Insect morphology
Insect Proteins
Insecta
Integument
Invertebrates
Physiology. Development
Protein Biosynthesis
protein composition
Proteins - immunology
Proteins - metabolism
Pupa - ultrastructure
Pupae
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Summary:Precise ultrastructural localization of Drosophila melanogaster pupal cuticle proteins (PCPs) was achieved by the immunogold labeling of frozen thin sections. PCPs were found in lamellate cuticle and intracellular vesicles but, curiously, were absent from the assembly zone of the cuticle. Antibodies that distinguish between the two classes of PCPs--low molecular weight (L-PCPs) and high molecular weight (H-PCPs)--revealed that the morphologically distinct outer lamellae contained L-PCPs and the inner lamellae contained H-PCPs. The sharp boundary between these two antigenic domains coincides with the transition from the outer to the inner lamellae, which in turn is correlated with the cessation of L-PCP synthesis and the initiation of H-PCP synthesis in response to 20-hydroxyecdysone. Hence, differences in protein composition are associated with differences in lamellar morphology.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.102.1.306