Distribution of acetylated alpha-tubulin in Physarum polycephalum

The expression and cytological distribution of acetylated alpha-tubulin was investigated in Physarum polycephalum. A monoclonal antibody specific for acetylated alpha-tubulin, 6-11B-1 (Piperno, G., and M. T. Fuller, 1985, J. Cell Biol., 101:2085-2094), was used to screen for this protein during thre...

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Bibliographic Details
Published in:The Journal of cell biology 1987-02, Vol.104 (2), p.303-309
Main Authors: Diggins, Maureen A., Dove, William F.
Format: Article
Language:English
Subjects:
Acetylation
Amoeba
Antibodies
anticorps monoclonal
anticuerpos monoclonales
Biological and medical sciences
Cells
champignon
Flagella
Flagella - ultrastructure
Fluorescent Antibody Technique
Fluorescent antibody techniques
Fundamental and applied biological sciences. Psychology
fungi
Gels
Microtubules
Mitosis
Mitotic spindle apparatus
monoclonal antibodies
Photomicrographs
Physarum - cytology
Physarum - growth & development
Physarum - metabolism
Physarum polycephalum
Protozoa
Systematics. Geographical distribution. Morphology. Cytology
Tubulin - metabolism
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Summary:The expression and cytological distribution of acetylated alpha-tubulin was investigated in Physarum polycephalum. A monoclonal antibody specific for acetylated alpha-tubulin, 6-11B-1 (Piperno, G., and M. T. Fuller, 1985, J. Cell Biol., 101:2085-2094), was used to screen for this protein during three different stages of the Physarum life cycle-the amoeba, the flagellate, and the plasmodium. Western blots of two-dimensional gels of amoebal and flagellate proteins reveal that this antibody recognizes the α3 tubulin isotype, which was previously shown to be formed by posttranslational modification (Green, L. L., and W. F. Dove, 1984, Mol. Cell. Biol., 4:1706-1711). Double-label immunofluorescence demonstrates that, in the flagellate, acetylated alpha-tubulin is localized in the flagella and flagellar cone. Similar experiments with amoebae interestingly reveal that only within the microtubule organizing center (MTOC) are there detectable amounts of acetylated alpha-tubulin. In contrast, the plasmodial stage gives no evidence for acetylated alpha-tubulin by Western blotting or by immunofluorescence.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.104.2.303