The Role of Extracellular Matrix in the Migration and Differentiation of Parietal Endoderm from Teratocarcinoma Embryoid Bodies

Embryoid bodies formed from teratocarcinoma stem cells differentiate an outer layer consisting of parietal and visceral endoderm or of visceral endoderm exclusively. We have previously shown that when these embryoid bodies are plated on collagen-coated substrates a parietal endoderm-like cell migrat...

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Bibliographic Details
Published in:The Journal of cell biology 1987-07, Vol.105 (1), p.441-448
Main Authors: Grabel, Laura B., Watts, Thomas D.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Adhesion
Cell aggregates
Cell coat. Cell surface
Cell Differentiation
Cell lines
Cell Movement
Cell structures and functions
Cells
Cells, Cultured
Collagen - physiology
Collagens
Embryoid bodies
Embryonic and Fetal Development
Embryonic stem cells
Embryos
Endoderm
Endoderm - pathology
Extracellular Matrix - physiology
Fibronectins - physiology
Fundamental and applied biological sciences. Psychology
Laminin - physiology
Mice
Molecular and cellular biology
Oligopeptides - pharmacology
Receptors, Fibronectin
Receptors, Immunologic - immunology
Receptors, Immunologic - physiology
Stem cells
Teratoma - pathology
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Summary:Embryoid bodies formed from teratocarcinoma stem cells differentiate an outer layer consisting of parietal and visceral endoderm or of visceral endoderm exclusively. We have previously shown that when these embryoid bodies are plated on collagen-coated substrates a parietal endoderm-like cell migrates onto the substrate, whereas all of the visceral endoderm remains associated with the stem cell mass, suggesting a role for substrate contact in parietal endoderm differentiation. We now identify fibronectin as the migration-promoting component in these cultures, and note that laminin and collagen type IV are 10-fold less effective at promoting both attachment and endoderm outgrowth. The RGDS tetrapeptide (arg-gly-asp-ser) from the cell attachment domain of fibronectin can specifically block attachment and outgrowth on both fibronectin- and laminin-coated substrates. In addition, the involvement of the 140-kD fibronectin receptor is demonstrated using an antibody directed against this molecule.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.105.1.441