Adhesion of Glycosaminoglycan-Deficient Chinese Hamster Ovary Cell Mutants to Fibronectin Substrata

We have examined the role of cell surface glycosaminoglycans in fibronectin-mediated cell adhesion by analyzing the adhesive properties of Chinese hamster ovary cell mutants deficient in glycosaminoglycans. The results of our study suggest that the absence of glycosaminoglycans does not affect the i...

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Bibliographic Details
Published in:The Journal of cell biology 1988-03, Vol.106 (3), p.945-952
Main Authors: LeBaron, Richard G., Esko, Jeffrey D., Woods, Anne, Johansson, Staffan, Höök, Magnus
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - ultrastructure
Animals
Biological and medical sciences
Cell Adhesion
Cell coat. Cell surface
Cell Line
Cell structures and functions
Cells
CHO cells
Cultured cells
Endothelial cells
Epithelial cells
Fibronectins - metabolism
Focal adhesions
Fundamental and applied biological sciences. Psychology
Glycosaminoglycans
Glycosaminoglycans - physiology
Hepatocytes
Microscopy, Interference
Molecular and cellular biology
Mutation
Proteoglycans
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Summary:We have examined the role of cell surface glycosaminoglycans in fibronectin-mediated cell adhesion by analyzing the adhesive properties of Chinese hamster ovary cell mutants deficient in glycosaminoglycans. The results of our study suggest that the absence of glycosaminoglycans does not affect the initial attachment and subsequent spreading of these cells on substrata composed of intact fibronectin or a fibronectin fragment containing the primary cell-binding domain. However, in contrast to wild-type cells, the glycosaminoglycan-deficient cells did not attach to substrata composed of a heparin-binding fibronectin fragment. Furthermore, the wild-type but not the glycosaminoglycan-deficient cells formed F-actin-containing stress fibers and focal adhesions on substrata composed of intact fibronectin. We propose, therefore, that cell surface proteoglycan(s) participate in the transmembrane linking of intracellular cytoskeletal components to extracellular matrix components which occurs in focal adhesions.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.106.3.945