Detection of a Specific Isoform of Alpha-Actinin with Antisera Directed against Dystrophin

We have characterized a protein immunologically related to dystrophin, the protein product of the Duchenne muscular dystrophy gene. We identify this related protein as a fast-twitch glycolytic isoform (mouse extensor digitorum longus-specific) of myofibrillar alpha-actinin. This specific isoform of...

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Bibliographic Details
Published in:The Journal of cell biology 1989-02, Vol.108 (2), p.503-510
Main Authors: Hoffman, Eric P., Watkins, Simon C., Slayter, Henry S., Kunkel, Louis M.
Format: Article
Language:English
Subjects:
Actinin - analysis
Analytical, structural and metabolic biochemistry
Animals
Antibodies
Antigens
antisera
Antiserum
Biological and medical sciences
Duchenne muscular dystrophy
Duchenne's muscular dystrophy
Dystrophin
Electrophoresis, Polyacrylamide Gel
Fast twitch muscle fibers
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Immune Sera
Immunoblotting
Immunology
Mice
Microscopy, Electron
Miscellaneous
Molecular Weight
Muscle Proteins - analysis
Muscle Proteins - immunology
Muscles
Muscles - analysis
Myofibrils - analysis
Protein isoforms
Proteins
Sequence Homology, Nucleic Acid
Skeletal muscle
Smooth muscle
Tissue Distribution
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Summary:We have characterized a protein immunologically related to dystrophin, the protein product of the Duchenne muscular dystrophy gene. We identify this related protein as a fast-twitch glycolytic isoform (mouse extensor digitorum longus-specific) of myofibrillar alpha-actinin. This specific isoform of alpha-actinin exhibits a more restricted pattern of expression in skeletal muscle than fast-twitch-specific isoforms of both myosin and Ca2+-ATPase. Our results provide evidence that dystrophin and myofibrillar alpha-actinin are related proteins, reinforcing the previous data concerning the sequence homologies noted between nonmuscle cytoskeletal alpha-actinin and dystrophin. In addition, we describe the first antisera directed against a specific myofibrillar skeletal muscle isoform of alpha-actinin.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.108.2.503