Transforming growth factor-beta 1: histochemical localization with antibodies to different epitopes

We have localized transforming growth factor-beta (TGF-beta) in many cells and tissues with immunohistochemical methods, using two polyclonal antisera raised to different synthetic preparations of a peptide corresponding to the amino-terminal 30 amino acids of TGF-beta 1. These two antibodies give d...

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Bibliographic Details
Published in:The Journal of cell biology 1989-02, Vol.108 (2), p.653-660
Main Authors: Flanders, K C, Thompson, N L, Cissel, D S, Van Obberghen-Schilling, E, Baker, C C, Kass, M E, Ellingsworth, L R, Roberts, A B, Sporn, M B
Format: Article
Language:English
Subjects:
Animals
Antibodies - immunology
Cattle
Colonic Neoplasms - analysis
Enzyme-Linked Immunosorbent Assay
Epitopes - immunology
Extracellular Matrix - analysis
Humans
Immunohistochemistry
Immunosorbent Techniques
Mice
Mice, Nude
Papilloma - analysis
Peptide Fragments - immunology
Protein Precursors - immunology
Radioimmunoassay
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha - analysis
Tumor Necrosis Factor-alpha - biosynthesis
Tumor Necrosis Factor-alpha - immunology
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Summary:We have localized transforming growth factor-beta (TGF-beta) in many cells and tissues with immunohistochemical methods, using two polyclonal antisera raised to different synthetic preparations of a peptide corresponding to the amino-terminal 30 amino acids of TGF-beta 1. These two antibodies give distinct staining patterns; the staining by anti-CC(1-30) is intracellular. This differential staining pattern is consistently observed in several systems, including cultured tumor cells; mouse embryonic, neonatal, and adult tissues; bovine fibropapillomas; and human colon carcinomas. The extracellular staining by anti-CC(1-30) partially resembles that seen with an antibody to fibronectin, suggesting that extracellular TGF-beta may be bound to matrix proteins. The intracellular staining by anti-LC(1-30) is similar to that seen with two other antibodies raised to peptides corresponding to either amino acids 266-278 of the TGF-beta 1 precursor sequence or to amino acids 50-75 of mature TGF-beta 1, suggesting that anti-LC(1-30) stains sites of TGF-beta synthesis. Results from RIA and ELISAs indicate that anti-LC(1-30) and anti-CC(1-30) recognize different epitopes of this peptide and of TGF-beta 1 itself.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.108.2.653