Subcellular Distribution of the 1,4-Dihydropyridine Receptor in Rabbit Skeletal Muscle in Situ: An Immunofluorescence and Immunocolloidal Gold-Labeling Study

• Published in: The Journal of cell biology 1989-07, Vol.109 (1), p.135-147
• Main Authors: Jorgensen, Annelise O., Amy C-Y. Shen, Arnold, Wayne, Leung, Albert T., Campbell, Kevin P.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibody Specificity; Biological and medical sciences; Calcium Channels - metabolism; Calcium-Transporting ATPases - metabolism; Caveolae; Cell Compartmentation; Cell receptors; Cell structures and functions; Dihydropyridines; Fluorescent Antibody Technique; Fluorescent antibody techniques; Fundamental and applied biological sciences. Psychology; Gold collolid; Immunohistochemistry; Molecular and cellular biology; Molecular Weight; Muscles; Muscles - metabolism; P branes; Rabbits; Receptors; Receptors, Nicotinic - metabolism; Sarcolemma; Sarcolemma - metabolism; Sarcoplasmic reticulum; Skeletal muscle
• ISSN: 0021-9525; 1540-8140
• DOI: 10.1083/jcb.109.1.135

The subcellular distribution of the 1,4-dihydropyridine receptor was determined in rabbit skeletal muscle in situ by immunofluorescence and immunoelectron microscopy. Longitudinal and transverse cryosections (5-8 μm) of rabbit gracilis muscle were labeled with monoclonal antibodies specific against either the α 1-subunit (170,000-D polypeptide) or the β-subunit (52,000-D polypeptide) of the 1,4-dihydropyridine receptor by immunofluorescence labeling. In longitudinal sections, specific labeling was present only near the interface between the A- and I-band regions of the sarcomeres. In transverse sections, specific labeling showed a hexagonal staining pattern within each myofiber however, the relative staining intensity of the type II (fast) fibers was judged to be three- to fourfold higher than that of the type I (slow) fibers. Specific immunofluorescence labeling of the sarcolemma was not observed in either longitudinal or transverse sections. These results are consistent with the idea that the α 1-subunit and the β-subunit of the purified 1,4-dihydropyridine receptor are densely distributed in the transverse tubular membrane. Immunoelectron microscopical localization with a monoclonal antibody to the α 1-subunit of the 1,4-dihydropyridine receptor showed that the 1,4-dihydropyridine receptor is densely distributed in the transverse tubular membrane. Approximately half of these were distributed in close proximity to the junctional region between the transverse tubules and the terminal cisternae. Specific labeling was also present in discrete foci in the subsarcolemmal region of the myofibers. The size and the nonrandom distribution of these foci in the subsarcolemmal region support the possibility that they correspond to invaginations from the sarcolemma called caveolae. In conclusion, our results demonstrate that the 1,4-dihydropyridine receptor in skeletal muscle is localized to the transverse tubular membrane and discrete foci in the subsarcolemmal region, possibly caveolae but absent from the lateral portion of the sarcolemma.