The GTP-Binding Protein Ypt1 Is Required for Transport in vitro: The Golgi Apparatus Is Defective in ypt1 Mutants

The YPT1 gene encodes a raslike, GTP-binding protein that is essential for growth of yeast cells. We show here that mutations in the ypt1 gene disrupt transport of carboxypeptidase Y to the vacuole in vivo and transport of pro-α-factor to a site of extensive glycosylation in the Golgi apparatus in v...

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Bibliographic Details
Published in:The Journal of cell biology 1989-09, Vol.109 (3), p.1015-1022
Main Authors: Bacon, Rebecca A., Salminen, Antti, Ruohola, Hannele, Novick, Peter, Ferro-Novick, Susan
Format: Article
Language:English
Subjects:
Alleles
Biological and medical sciences
Calcium
Calcium - metabolism
Carboxypeptidases - genetics
Cathepsin A
Cell growth
Cell physiology
Cells
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
Gels
Genes
Genes, Fungal
Genetic mutation
Glycosylation
Golgi apparatus
Golgi Apparatus - physiology
GTP-Binding Proteins - genetics
GTP-Binding Proteins - physiology
Membrane and intracellular transports
Molecular and cellular biology
Mutation
Plasmids
Protein Precursors - genetics
Protein Processing, Post-Translational
Proteins - genetics
rab GTP-Binding Proteins
Saccharomyces
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Secretory pathway
Species Specificity
Vacuoles - metabolism
Yeasts
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Description
Summary:The YPT1 gene encodes a raslike, GTP-binding protein that is essential for growth of yeast cells. We show here that mutations in the ypt1 gene disrupt transport of carboxypeptidase Y to the vacuole in vivo and transport of pro-α-factor to a site of extensive glycosylation in the Golgi apparatus in vitro. Two different ypt1 mutations result in loss of function of the Golgi complex without affecting the activity of the endoplasmic reticulum or soluble components required for in vitro transport. The function of the mutant Golgi apparatus can be restored by preincubation with wild-type cytosol. The transport defect observed in vitro cannot be overcome by addition of Ca++ to the reaction mixture. We have also established genetic interactions between ypt1 and a subset of the other genes required for transport to and through the Golgi apparatus.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.109.3.1015