A Dictyostelium discoideum Mutant That Missorts and Oversecretes Lysosomal Enzyme Precursors Is Defective in Endocytosis

A mutant strain of Dictyostelium discoideum, HMW570, oversecretes several lysosomal enzyme activities during growth. Using a radiolable pulse-chase protocol, we followed the synthesis and secretion of two of these enzymes, α-mannosidase and β-glucosidase. A few hours into the chase period, HMW570 ha...

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Bibliographic Details
Published in:The Journal of cell biology 1989-10, Vol.109 (4), p.1445-1456
Main Authors: Ebert, David L., Freeze, Hudson H., Richardson, Jan, Dimond, Randall L., Cardelli, James A.
Format: Article
Language:English
Subjects:
alpha -mannosidase
beta -glucosidase
beta-Glucosidase - biosynthesis
beta-Glucosidase - genetics
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Cell Fractionation
Cells
Centrifugation
Dictyostelium - enzymology
Dictyostelium - genetics
Dictyostelium - physiology
Endocytosis
Endosomes
Enzyme Precursors - biosynthesis
Enzyme Precursors - genetics
Enzymes
Fundamental and applied biological sciences. Psychology
Glucosidases - genetics
Kinetics
lysosomal enzymes
Lysosomes
Lysosomes - enzymology
Mannosidases - biosynthesis
Mannosidases - genetics
Mutation
Oligosaccharides
Oligosaccharides - isolation & purification
Oligosaccharides - metabolism
Protein Processing, Post-Translational
Protozoa
Receptors
Secretion
Stem cells
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Description
Summary:A mutant strain of Dictyostelium discoideum, HMW570, oversecretes several lysosomal enzyme activities during growth. Using a radiolable pulse-chase protocol, we followed the synthesis and secretion of two of these enzymes, α-mannosidase and β-glucosidase. A few hours into the chase period, HMW570 had secreted 95% of its radiolabeled α-mannosidase and 86% of its radiolabeled β-glucosidase as precursor polypeptides compared to the secretion of
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.109.4.1445