The Extracellular Matrix of Volvox carteri: Molecular Structure of the Cellular Compartment

The extracellular matrix (ECM) of Volvox contains insoluble fibrous layers that surround individual cells at a distance to form contiguous cellular compartments. Using immunological techniques, we identified a sulfated surface glycoprotein (SSG 185) as the monomeric precursor of this substructure wi...

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Bibliographic Details
Published in:The Journal of cell biology 1989-12, Vol.109 (6), p.3493-3501
Main Authors: Ertl, Hans, Mengele, Reiner, Wenzl, Stephan, Engel, Jürgen, Sumper, Manfred
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Analytical, structural and metabolic biochemistry
Antibodies, Monoclonal
Base Sequence
Biological and medical sciences
Carbohydrate Metabolism
Cell Compartmentation
Cell walls
Chlorophyta - genetics
Chlorophyta - ultrastructure
Cloning, Molecular
Complementary DNA
DNA - genetics
Extracellular Matrix - ultrastructure
Extracellular Matrix Proteins
Female
Freshwater
Fundamental and applied biological sciences. Psychology
Gene Library
Glycopeptides
Glycoproteins
Glycoproteins - genetics
Glycoproteins - metabolism
Glycoproteins - ultrastructure
Methylation
Molecular Sequence Data
Molecular structure
Peptide Fragments - metabolism
Peptide Hydrolases
Polymers
Polysaccharides
Proteins
Sulfates
Sulfates - metabolism
Volvox carteri
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Description
Summary:The extracellular matrix (ECM) of Volvox contains insoluble fibrous layers that surround individual cells at a distance to form contiguous cellular compartments. Using immunological techniques, we identified a sulfated surface glycoprotein (SSG 185) as the monomeric precursor of this substructure within the ECM. The primary structure of the SSG 185 polypeptide chain has been derived from cDNA and genomic DNA. A central domain of the protein, 80 amino acid residues long, consists almost exclusively of hydroxyproline residues. The chemical structure of the highly sulfated polysaccharide covalently attached to SSG 185 has been determined by permethylation analysis. As revealed by EM, SSG 185 is a rod-shaped molecule with a 21-nm-long polysaccharide strand protruding from its central region. The chemical nature of the cross-links between SSG 185 monomers is discussed.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.109.6.3493