Evidence for the Presence of a Proton Pump of the Vacuolar H+- ATPase Type in the Ruffled Borders of Osteoclasts

Microsomal membrane vesicles prepared either from chicken medullary bone or isolated osteoclasts were shown to have ATP-dependent H+-transport activity. This activity was N-ethylmaleimide-sensitive but resistant to oligomycin and orthovanadate, suggesting a vacuolar-type ATPase. Furthermore, immunol...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 1990-09, Vol.111 (3), p.1305-1311
Main Authors: Väänänen, H. K., E.-K. Karhukorpi, Sundquist, K., Wallmark, B., Roininen, I., Hentunen, T., Tuukkanen, J., Lakkakorpi, P.
Format: Article
Language:English
Subjects:
Adenosine triphosphatases
Animals
Antibodies
Biological and medical sciences
Blotting, Western
Bones
Cell membranes
Cell physiology
Cells
Chickens
Cross Reactions
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Kidney cells
Membrane and intracellular transports
Microscopy, Electron
Microsomes
Microsomes - enzymology
Molecular and cellular biology
Molecular Weight
Neurospora crassa - enzymology
Osteoclasts
Osteoclasts - enzymology
P branes
Parathyroid Hormone - physiology
Proton pumps
Proton-Translocating ATPases - analysis
Protons
Rats
Stomach - enzymology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Microsomal membrane vesicles prepared either from chicken medullary bone or isolated osteoclasts were shown to have ATP-dependent H+-transport activity. This activity was N-ethylmaleimide-sensitive but resistant to oligomycin and orthovanadate, suggesting a vacuolar-type ATPase. Furthermore, immunological cross-reactivity of 60- and 70-kD osteoclast membrane antigens with Neurospora crassa vacuolar ATPase was observed when analyzed by immunoblotting. Same antibodies labeled only osteoclasts in chicken and rat bone in immunohistochemistry. Immunoelectronmicroscopy localized these antigens in apical membranes of rat osteoclasts and kidney intercalated cells of inner stripe of outer medulla. Pretreatment of animals with parathyroid hormone enhanced the immunoreaction in the apical membranes of osteoclasts. No immunoreaction was seen in osteoclasts when antibodies against gastric H+, K+- ATPase were used. These results suggest that osteoclast resorbs bone by secreting protons through vacuolar H+- ATPase.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.111.3.1305