Satellite Cell Proliferation in the Adult Rat Trigeminal Ganglion Results from the Release of a Mitogenic Protein from Explanted Sensory Neurons

Explant of trigeminal ganglia neurons in adult rats induces perineuronal glial proliferation of primarily satellite cells as opposed to Schwann cells. This proliferation begins at 15 h after explant culture and by 27 h there is a significant increase in glial proliferation as measured by scintillati...

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Bibliographic Details
Published in:The Journal of cell biology 1994-03, Vol.124 (6), p.1005-1015
Main Authors: Joseph Y. M. Wen, Morshead, Cindi M., van der Kooy, Derek
Format: Article
Language:English
Subjects:
Animals
Artificial satellites
Biological and medical sciences
Cell cycle, cell proliferation
Cell Division
Cell growth
Cell physiology
Cellular biology
Culture Media, Conditioned
Culture Techniques
Cultured cells
Cycloheximide - pharmacology
Fundamental and applied biological sciences. Psychology
Ganglia
Hot Temperature
Male
Mitogens
Mitogens - metabolism
Molecular and cellular biology
Nerve Growth Factors - pharmacology
Nerve Tissue Proteins - pharmacology
Neuroglia
Neuroglia - cytology
Neurons
Neurons, Afferent - metabolism
Neurons, Afferent - physiology
Protein Biosynthesis
Protein synthesis
Rats
Rodents
S Phase
Schwann cells
Trigeminal ganglion
Trigeminal Ganglion - cytology
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Summary:Explant of trigeminal ganglia neurons in adult rats induces perineuronal glial proliferation of primarily satellite cells as opposed to Schwann cells. This proliferation begins at 15 h after explant culture and by 27 h there is a significant increase in glial proliferation as measured by scintillation counts of [3H]thymidine. Blocking protein synthesis between 0 and 3.5 h after explant culture (early) results in an enhanced proliferative response, while blocking protein synthesis between 3.5 and 7 h (late) causes a complete block of the proliferative response assessed at 27 h. Conditioned media experiments demonstrate that both the mitogenic and inhibitory signals are diffusible and heat labile. Finally, the addition of neurotrophic factors to rescue injured ganglionic neurons attenuates the proliferative glial response suggesting that injured neurons produce and release signals that induce glial proliferation.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.124.6.1005