Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae

In many eucaryotic cells, the midzone of the mitotic spindle forms a distinct structure containing a specific set of proteins. We have isolated ASE1, a gene encoding a component of the Saccharomyces cerevisiae spindle midzone. Strains lacking both ASE1 and BIK1, which encodes an S. cerevisiae microt...

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Bibliographic Details
Published in:The Journal of cell biology 1995-09, Vol.130 (6), p.1373-1385
Main Authors: Pellman, D. (The Whitehead Institute for Biomedical Research, Cambridge, MA.), Bagget, M, Tu, H, Fink, G.R
Format: Article
Language:English
Subjects:
Anaphase
Bacteriology
Base Sequence
Cell cycle
Cell Division
Cell nucleus
Cells
Cellular biology
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
ESTRUCTURA CELULAR
Fungal Proteins - genetics
Fungal Proteins - metabolism
GENE
GENES
Genetics
LOCI
LOCUS
Microscopy, Electron
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Microtubules
MITOSE
MITOSIS
Mitotic spindle apparatus
Molecular Sequence Data
MUTACION
Mutagenesis, Site-Directed
MUTANT
MUTANTES
MUTATION
ORGANITE CELLULAIRE
ORGANULOS CITOPLASMICOS
Plasmids
PROTEINAS AGLUTINANTES
PROTEINE DE LIAISON
Proteins
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae - ultrastructure
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
Spindle pole body
STRUCTURE CELLULAIRE
Yeasts
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Summary:In many eucaryotic cells, the midzone of the mitotic spindle forms a distinct structure containing a specific set of proteins. We have isolated ASE1, a gene encoding a component of the Saccharomyces cerevisiae spindle midzone. Strains lacking both ASE1 and BIK1, which encodes an S. cerevisiae microtubule-associated protein, are inviable. The analysis of the phenotype of a bik1 ase1 conditional double mutant suggests that BIK1 and ASE1 are not required for the assembly of a bipolar spindle, but are essential for anaphase spindle elongation. The steady-state levels of Ase1p are regulated in a manner that is consistent with a function during anaphase: they are low in G1, accumulate to maximal levels after S phase and then drop as cells exit mitosis. Components of the spindle midzone may therefore be required in vivo for anaphase spindle movement. Additionally, anaphase spindle movement may depend on a dedicated set of genes whose expression is induced at G2/M
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.130.6.1373