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Heparin Is an Adhesive Ligand for the Leukocyte Integrin Mac-1 (CD11b/CD18)

Previous studies have demonstrated that the leukocyte integrin Mac-1 adheres to several cell surface and soluble ligands including intercellular adhesion molecule-1, fibrinogen, iC3b, and factor X. However, experiments with Mac-1-expressing transfectants, purified Mac-1, and mAbs to Mac-1 indicate t...

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Published in:	The Journal of cell biology 1995-09, Vol.130 (6), p.1473-1482
Main Authors:	Diamond, Michael S., Alon, Ronen, Parkos, Charles A., Quinn, Mark T., Springer, Timothy A.
Format:	Article
Language:	English
Subjects:	Adhesives Animals Cell Adhesion Cellular biology CHO Cells Circulatory system Cricetinae Endothelial cells Fibrosis Heparin Heparin - metabolism Humans Integrins Leukocytes Ligands Macrophage-1 Antigen - metabolism Middle Aged Neutrophils Neutrophils - metabolism Sensitivity and Specificity Shear stress Sulfates
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cited_by	cdi_FETCH-LOGICAL-c499t-e1bfe2d1697e6dc56f754dabdf5ceac0f2661bc880654c00b821996eac47a5043
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container_end_page	1482
container_issue	6
container_start_page	1473
container_title	The Journal of cell biology
container_volume	130
creator	Diamond, Michael S. Alon, Ronen Parkos, Charles A. Quinn, Mark T. Springer, Timothy A.
description	Previous studies have demonstrated that the leukocyte integrin Mac-1 adheres to several cell surface and soluble ligands including intercellular adhesion molecule-1, fibrinogen, iC3b, and factor X. However, experiments with Mac-1-expressing transfectants, purified Mac-1, and mAbs to Mac-1 indicate the existence of additional ligands. In this paper, we demonstrate a direct interaction between Mac-1 and heparan sulfate glycans. Heparin affinity resins immunoprecipitate Mac-1, and neutrophils and transfectant cells that express Mac-1 bind to heparin and heparan sulfate, but not to other sulfated glycosaminoglycans. Inhibition studies with mAbs and chemically modified forms of heparin suggest the I domain as a recognition site on Mac-1 for heparin, and suggest that either N- or O-sulfation is sufficient for heparin to bind efficiently to Mac-1. Under conditions of continuous flow in which heparins and E-selectin are cosubstrates, neutrophils tether to E-selectin and form firm adhesions through a Mac-1-heparin interaction.
doi_str_mv	10.1083/jcb.130.6.1473
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source	JSTOR Archival Journals and Primary Sources Collection
subjects	Adhesives Animals Cell Adhesion Cellular biology CHO Cells Circulatory system Cricetinae Endothelial cells Fibrosis Heparin Heparin - metabolism Humans Integrins Leukocytes Ligands Macrophage-1 Antigen - metabolism Middle Aged Neutrophils Neutrophils - metabolism Sensitivity and Specificity Shear stress Sulfates
title	Heparin Is an Adhesive Ligand for the Leukocyte Integrin Mac-1 (CD11b/CD18)
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