Preservation of Staphylococcus aureus with unstable antibiotic resistance by drying

Cultures of Staphylococcus aureus showing unstable resistance to kanamycin, streptomycin, lincomycin and penicillin have been preserved by drying. Particular attention has been paid to organisms showing linked resistance to pairs of these antibiotics. Recoveries of viable cells from the desiccates w...

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Bibliographic Details
Published in:The Journal of hygiene 1973-06, Vol.71 (2), p.411-416
Main Authors: Annear, D. I., Grubb, W. B.
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Bacteria
Bacteriological Techniques
Cell Count
Chemical suspensions
Cultural preservation
Drying
Genetics, Microbial
Kanamycin - pharmacology
Lincomycin - pharmacology
Penicillin
Penicillin Resistance
Penicillins - pharmacology
Preservation, Biological
Room temperature
Silica gel
Staphylococcus - drug effects
Staphylococcus aureus
Streptomycin - pharmacology
Time Factors
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Summary:Cultures of Staphylococcus aureus showing unstable resistance to kanamycin, streptomycin, lincomycin and penicillin have been preserved by drying. Particular attention has been paid to organisms showing linked resistance to pairs of these antibiotics. Recoveries of viable cells from the desiccates was high and the proportion of resistant and sensitive cells was maintained both during storage and during heating. The preservation of the organisms on ceramic beads stored in air over silica gel promises to be an effective and convenient method for maintaining staphylococcal desiccates during studies of unstable antibiotic resistance.
ISSN:0022-1724
2396-8184
DOI:10.1017/S0022172400022853