A Mouse Cytoplasmic Exoribonuclease (mXRN1p) with Preference for G4 Tetraplex Substrates

Exoribonucleases are important enzymes for the turnover of cellular RNA species. We have isolated the first mammalian cDNA from mouse demonstrated to encode a 5′-3′ exoribonuclease. The structural conservation of the predicted protein and complementation data in Saccharomyces cerevisiae suggest a ro...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 1997-02, Vol.136 (4), p.761-773
Main Authors: Bashkirov, Vladimir I., Scherthan, Harry, Solinger, Jachen A., Buerstedde, Jean-Marie, Heyer, Wolf-Dietrich
Format: Article
Language:English
Subjects:
Animals
Antibodies
Cell lines
Cells
Cellular biology
Complementary DNA
Cytoplasm - enzymology
Cytoplasm - metabolism
Deoxyribonucleases - genetics
Deoxyribonucleic acid
DNA
DNA, Complementary - chemistry
DNA, Complementary - isolation & purification
DNA, Complementary - physiology
Enzymes
Exoribonucleases - genetics
Exoribonucleases - metabolism
Fungal Proteins - genetics
Guanine - metabolism
Messenger RNA
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Plasmids
Ribonucleic acid
RNA
RNA - metabolism
Rodents
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
Substrate Specificity
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Exoribonucleases are important enzymes for the turnover of cellular RNA species. We have isolated the first mammalian cDNA from mouse demonstrated to encode a 5′-3′ exoribonuclease. The structural conservation of the predicted protein and complementation data in Saccharomyces cerevisiae suggest a role in cytoplasmic mRNA turnover and pre-rRNA processing similar to that of the major cytoplasmic exoribonuclease Xrn1p in yeast. Therefore, a key component of the mRNA decay system in S. cerevisiae has been conserved in evolution from yeasts to mammals. The purified mouse protein (mXRN1p) exhibited a novel substrate preference for G4 RNA tetraplex-containing substrates demonstrated in binding and hydrolysis experiments. mXRN1p is the first RNA turnover function that has been localized in the cytoplasm of mammalian cells. mXRN1p was distributed in small granules and was highly enriched in discrete, prominent foci. The specificity of mXRN1p suggests that RNAs containing G4 tetraplex structures may occur in vivo and may have a role in RNA turnover.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.136.4.761