A Presumptive Developmental Role for a Sea Urchin Cyclin B Splice Variant

We show that a splice variant-derived cyclin B is produced in sea urchin oocytes and embryos. This splice variant protein lacks highly conserved sequences in the COOH terminus of the protein. It is found strikingly abundant in growing oocytes and cells committed to differentiation during embryogenes...

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Bibliographic Details
Published in:The Journal of cell biology 1998-01, Vol.140 (2), p.283-293
Main Authors: Lozano, Jean-Claude, Schatt, Philippe, Marquès, François, Peaucellier, Gérard, Fort, Philippe, Féral, Jean-Pierre, Genevière, Anne-Marie, Picard, André
Format: Article
Language:English
Subjects:
Alternative Splicing
Amino Acid Sequence
Amino acids
Animals
Biochemistry, Molecular Biology
CDC2 Protein Kinase
CDC2 Protein Kinase - metabolism
Cell cycle
Cell division
Cellular Biology
Cloning, Molecular
Complementary DNA
Cyclin B
Cyclin B - genetics
Cyclin B - physiology
Cyclins
Development Biology
Eggs
Embryology and Organogenesis
Embryos
Invertebrates
Life Sciences
Marine
Maturation-Promoting Factor
Maturation-Promoting Factor - metabolism
Messenger RNA
Microinjections
Mitosis
Molecular biology
Molecular Sequence Data
Oocytes
Oocytes - cytology
Polymerase chain reaction
Proteins
RNA, Messenger
RNA, Messenger - chemistry
Sea Urchins
Sea Urchins - embryology
Sequence Alignment
Strongylocentrotus purpuratus
Subcellular Processes
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Summary:We show that a splice variant-derived cyclin B is produced in sea urchin oocytes and embryos. This splice variant protein lacks highly conserved sequences in the COOH terminus of the protein. It is found strikingly abundant in growing oocytes and cells committed to differentiation during embryogenesis. Cyclin B splice variant (CBsv) protein associates weakly in the cell with Xenopus cdc2 and with budding yeast CDC28p. In contrast to classical cyclin B, CBsv very poorly complements a triple CLN deletion in budding yeast, and its microinjection prevents an initial step in MPF activation, leading to an important delay in oocyte meiosis reinitiation. CBsv microinjection in fertilized eggs induces cell cycle delay and abnormal development. We assume that CBsv is produced in growing oocytes to keep them in prophase, and during embryogenesis to slow down cell cycle in cells that will be committed to differentiation.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.140.2.283