The anaerobic bacterial flora of the mouse cecum

Refinements of the anaerobic technique such as the utilization of prereduced media and the removal of traces of oxygen from the gases used by passage through a hot copper oven resulted in quantitative and qualitative improvements in the recovery of anaerobic bacteria from the cecum of SPF mice. The...

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Bibliographic Details
Published in:The Journal of experimental medicine 1970-08, Vol.132 (2), p.251-260
Main Authors: Gordon, J H, Dubos, R
Format: Article
Language:English
Subjects:
Air
Animals
Bacteriological Techniques
Cecum - microbiology
Clostridium - isolation & purification
Eubacterium - isolation & purification
Fusobacterium - isolation & purification
Mice
Microscopy, Phase-Contrast
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Summary:Refinements of the anaerobic technique such as the utilization of prereduced media and the removal of traces of oxygen from the gases used by passage through a hot copper oven resulted in quantitative and qualitative improvements in the recovery of anaerobic bacteria from the cecum of SPF mice. The commonest morphologic types of bacteria in the mouse cecum were tapered rods. These characteristically gave "speckled" colonies on agar plates. Those identified were species of the genera Fusobacterium, Eubacterium, and Clostridium. Several of the morphologic types seen with phase microscopy still could not be cultivated. The implications of these findings for the study of the intestinal flora are discussed.
ISSN:0022-1007
1540-9538
DOI:10.1084/jem.132.2.251