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The Dynamic Nuclear Redistribution of an hnRNP K-Homologous Protein during Drosophila Embryo Development and Heat Shock. Flexibility of Transcription Sites in Vivo

The Drosophila protein Hrb57A has sequence homology to mammalian heterogenous nuclear ribonucleoprotein (hnRNP) K proteins. Its in vivo distribution has been studied at high resolution by confocal laser scanning microscopy (CLSM) in embryos injected with fluorescently labeled monoclonal antibody. In...

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Published in:The Journal of cell biology 1997-04, Vol.137 (2), p.291-303
Main Authors: Buchenau, Peter, Saumweber, Harald, Arndt-Jovin, Donna J.
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Saumweber, Harald
Arndt-Jovin, Donna J.
description The Drosophila protein Hrb57A has sequence homology to mammalian heterogenous nuclear ribonucleoprotein (hnRNP) K proteins. Its in vivo distribution has been studied at high resolution by confocal laser scanning microscopy (CLSM) in embryos injected with fluorescently labeled monoclonal antibody. Injection of antibody into living embryos had no apparent deleterious effects on further development. Furthermore, the antibody-protein complex could be observed for more than 7 cell cycles in vivo, revealing a dynamic redistribution from the nucleus to cytoplasm at each mitosis from blastoderm until hatching. The evaluation of two- and three-dimensional CLSM data sets demonstrated important differences in the localization of the protein in the nuclei of living compared to fixed embryos. The Hrb57A protein was recruited to the 93D locus upon heat shock and thus serves as an in vivo probe for the activity of the gene in diploid cells of the embryo. Observations during heat shock revealed considerable mobility within interphase nuclei of this transcription site. Furthermore, the reinitiation as well as the down regulation of transcriptional loci in vivo during the recovery from heat shock could be followed by the rapid redistribution of the hnRNP K during stress recovery. These data are incompatible with a model of the interphase nucleus in which transcription complexes are associated with a rigid nuclear matrix.
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Flexibility of Transcription Sites in Vivo</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1997-04-21</date><risdate>1997</risdate><volume>137</volume><issue>2</issue><spage>291</spage><epage>303</epage><pages>291-303</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>The Drosophila protein Hrb57A has sequence homology to mammalian heterogenous nuclear ribonucleoprotein (hnRNP) K proteins. Its in vivo distribution has been studied at high resolution by confocal laser scanning microscopy (CLSM) in embryos injected with fluorescently labeled monoclonal antibody. Injection of antibody into living embryos had no apparent deleterious effects on further development. Furthermore, the antibody-protein complex could be observed for more than 7 cell cycles in vivo, revealing a dynamic redistribution from the nucleus to cytoplasm at each mitosis from blastoderm until hatching. 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ispartof The Journal of cell biology, 1997-04, Vol.137 (2), p.291-303
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subjects Animals
Antibodies
Antibodies, Monoclonal
Cell Nucleus - metabolism
Cells
Chromosomes
Drosophila
Drosophila melanogaster
Drosophila melanogaster - embryology
Embryo, Nonmammalian
Embryos
Gene Expression Regulation, Developmental
Genes
Genetic loci
Heat-Shock Response - physiology
Heterogeneous nuclear ribonucleoproteins
Heterogeneous-Nuclear Ribonucleoprotein K
Interphase
Proteins
Ribonucleoproteins - metabolism
RNA
RNA, Messenger - analysis
Shock heating
Transcription, Genetic - physiology
Transcriptional Activation
title The Dynamic Nuclear Redistribution of an hnRNP K-Homologous Protein during Drosophila Embryo Development and Heat Shock. Flexibility of Transcription Sites in Vivo
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