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The Dynamic Nuclear Redistribution of an hnRNP K-Homologous Protein during Drosophila Embryo Development and Heat Shock. Flexibility of Transcription Sites in Vivo
The Drosophila protein Hrb57A has sequence homology to mammalian heterogenous nuclear ribonucleoprotein (hnRNP) K proteins. Its in vivo distribution has been studied at high resolution by confocal laser scanning microscopy (CLSM) in embryos injected with fluorescently labeled monoclonal antibody. In...
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Published in: | The Journal of cell biology 1997-04, Vol.137 (2), p.291-303 |
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description | The Drosophila protein Hrb57A has sequence homology to mammalian heterogenous nuclear ribonucleoprotein (hnRNP) K proteins. Its in vivo distribution has been studied at high resolution by confocal laser scanning microscopy (CLSM) in embryos injected with fluorescently labeled monoclonal antibody. Injection of antibody into living embryos had no apparent deleterious effects on further development. Furthermore, the antibody-protein complex could be observed for more than 7 cell cycles in vivo, revealing a dynamic redistribution from the nucleus to cytoplasm at each mitosis from blastoderm until hatching. The evaluation of two- and three-dimensional CLSM data sets demonstrated important differences in the localization of the protein in the nuclei of living compared to fixed embryos. The Hrb57A protein was recruited to the 93D locus upon heat shock and thus serves as an in vivo probe for the activity of the gene in diploid cells of the embryo. Observations during heat shock revealed considerable mobility within interphase nuclei of this transcription site. Furthermore, the reinitiation as well as the down regulation of transcriptional loci in vivo during the recovery from heat shock could be followed by the rapid redistribution of the hnRNP K during stress recovery. These data are incompatible with a model of the interphase nucleus in which transcription complexes are associated with a rigid nuclear matrix. |
doi_str_mv | 10.1083/jcb.137.2.291 |
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The evaluation of two- and three-dimensional CLSM data sets demonstrated important differences in the localization of the protein in the nuclei of living compared to fixed embryos. The Hrb57A protein was recruited to the 93D locus upon heat shock and thus serves as an in vivo probe for the activity of the gene in diploid cells of the embryo. Observations during heat shock revealed considerable mobility within interphase nuclei of this transcription site. Furthermore, the reinitiation as well as the down regulation of transcriptional loci in vivo during the recovery from heat shock could be followed by the rapid redistribution of the hnRNP K during stress recovery. These data are incompatible with a model of the interphase nucleus in which transcription complexes are associated with a rigid nuclear matrix.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.137.2.291</identifier><identifier>PMID: 9128243</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Animals ; Antibodies ; Antibodies, Monoclonal ; Cell Nucleus - metabolism ; Cells ; Chromosomes ; Drosophila ; Drosophila melanogaster ; Drosophila melanogaster - embryology ; Embryo, Nonmammalian ; Embryos ; Gene Expression Regulation, Developmental ; Genes ; Genetic loci ; Heat-Shock Response - physiology ; Heterogeneous nuclear ribonucleoproteins ; Heterogeneous-Nuclear Ribonucleoprotein K ; Interphase ; Proteins ; Ribonucleoproteins - metabolism ; RNA ; RNA, Messenger - analysis ; Shock heating ; Transcription, Genetic - physiology ; Transcriptional Activation</subject><ispartof>The Journal of cell biology, 1997-04, Vol.137 (2), p.291-303</ispartof><rights>Copyright 1997 The Rockefeller University Press</rights><rights>Copyright Rockefeller University Press Apr 21, 1997</rights><rights>1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-ac51e48febfeb224d549204a41ad5e47846352c870115d168e88f3908a1cc1543</citedby><cites>FETCH-LOGICAL-c527t-ac51e48febfeb224d549204a41ad5e47846352c870115d168e88f3908a1cc1543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1618011$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1618011$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,881,27898,27899,58210,58443</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9128243$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Buchenau, Peter</creatorcontrib><creatorcontrib>Saumweber, Harald</creatorcontrib><creatorcontrib>Arndt-Jovin, Donna J.</creatorcontrib><title>The Dynamic Nuclear Redistribution of an hnRNP K-Homologous Protein during Drosophila Embryo Development and Heat Shock. Flexibility of Transcription Sites in Vivo</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>The Drosophila protein Hrb57A has sequence homology to mammalian heterogenous nuclear ribonucleoprotein (hnRNP) K proteins. Its in vivo distribution has been studied at high resolution by confocal laser scanning microscopy (CLSM) in embryos injected with fluorescently labeled monoclonal antibody. Injection of antibody into living embryos had no apparent deleterious effects on further development. Furthermore, the antibody-protein complex could be observed for more than 7 cell cycles in vivo, revealing a dynamic redistribution from the nucleus to cytoplasm at each mitosis from blastoderm until hatching. The evaluation of two- and three-dimensional CLSM data sets demonstrated important differences in the localization of the protein in the nuclei of living compared to fixed embryos. The Hrb57A protein was recruited to the 93D locus upon heat shock and thus serves as an in vivo probe for the activity of the gene in diploid cells of the embryo. Observations during heat shock revealed considerable mobility within interphase nuclei of this transcription site. Furthermore, the reinitiation as well as the down regulation of transcriptional loci in vivo during the recovery from heat shock could be followed by the rapid redistribution of the hnRNP K during stress recovery. These data are incompatible with a model of the interphase nucleus in which transcription complexes are associated with a rigid nuclear matrix.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal</subject><subject>Cell Nucleus - metabolism</subject><subject>Cells</subject><subject>Chromosomes</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - embryology</subject><subject>Embryo, Nonmammalian</subject><subject>Embryos</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes</subject><subject>Genetic loci</subject><subject>Heat-Shock Response - physiology</subject><subject>Heterogeneous nuclear ribonucleoproteins</subject><subject>Heterogeneous-Nuclear Ribonucleoprotein K</subject><subject>Interphase</subject><subject>Proteins</subject><subject>Ribonucleoproteins - metabolism</subject><subject>RNA</subject><subject>RNA, Messenger - analysis</subject><subject>Shock heating</subject><subject>Transcription, Genetic - physiology</subject><subject>Transcriptional Activation</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFUk1v0zAAtRBolMGRG0gWB24JtmPHzgUJrduKmMa0Fa6W4zitS2IHO6no79kfxaXV-LggWfLhPb33_PwAeIlRjpEo3m10neOC5yQnFX4EZphRlAlM0WMwQ4jgrGKEPQXPYtwghCinxQk4qTARhBYzcL9cGzjfOdVbDa8n3RkV4K1pbByDrafRegd9C5WDa3d7fQM_ZQvf-86v_BThTfCjsQ42U7BuBefBRz-sbafgeV-HnYdzszWdH3rjxiTRwIVRI7xbe_0thxed-WFr29lxt3dYBuWiDnb4ZXlnRxNhkv5qt_45eNKqLpoXx_sUfLk4X54tsqvPlx_PPlxlmhE-ZkozbKhoTZ0OIbRhtCKIKopVwwzlgpYFI1pwhDFrcCmMEG1RIaGw1qm14hS8P-gOU92bRqfUQXVyCLZXYSe9svJvxNm1XPmtJLioOEdJ4O1RIPjvk4mj7G3UpuuUM6kvyUXFi7Ji_yXiEmHGxD7Sm3-IGz8Fl1pIphxxgcoykbIDSacPiMG0D5ExkvuNyLQRmTYiiUwbSfzXf77zgX0cRcJfHfBNHH34LVZikaorfgI63MNn</recordid><startdate>19970421</startdate><enddate>19970421</enddate><creator>Buchenau, Peter</creator><creator>Saumweber, Harald</creator><creator>Arndt-Jovin, Donna J.</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7SS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970421</creationdate><title>The Dynamic Nuclear Redistribution of an hnRNP K-Homologous Protein during Drosophila Embryo Development and Heat Shock. Flexibility of Transcription Sites in Vivo</title><author>Buchenau, Peter ; Saumweber, Harald ; Arndt-Jovin, Donna J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527t-ac51e48febfeb224d549204a41ad5e47846352c870115d168e88f3908a1cc1543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>Cell Nucleus - metabolism</topic><topic>Cells</topic><topic>Chromosomes</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - embryology</topic><topic>Embryo, Nonmammalian</topic><topic>Embryos</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genes</topic><topic>Genetic loci</topic><topic>Heat-Shock Response - physiology</topic><topic>Heterogeneous nuclear ribonucleoproteins</topic><topic>Heterogeneous-Nuclear Ribonucleoprotein K</topic><topic>Interphase</topic><topic>Proteins</topic><topic>Ribonucleoproteins - metabolism</topic><topic>RNA</topic><topic>RNA, Messenger - analysis</topic><topic>Shock heating</topic><topic>Transcription, Genetic - physiology</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buchenau, Peter</creatorcontrib><creatorcontrib>Saumweber, Harald</creatorcontrib><creatorcontrib>Arndt-Jovin, Donna J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buchenau, Peter</au><au>Saumweber, Harald</au><au>Arndt-Jovin, Donna J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Dynamic Nuclear Redistribution of an hnRNP K-Homologous Protein during Drosophila Embryo Development and Heat Shock. Flexibility of Transcription Sites in Vivo</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1997-04-21</date><risdate>1997</risdate><volume>137</volume><issue>2</issue><spage>291</spage><epage>303</epage><pages>291-303</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>The Drosophila protein Hrb57A has sequence homology to mammalian heterogenous nuclear ribonucleoprotein (hnRNP) K proteins. Its in vivo distribution has been studied at high resolution by confocal laser scanning microscopy (CLSM) in embryos injected with fluorescently labeled monoclonal antibody. Injection of antibody into living embryos had no apparent deleterious effects on further development. Furthermore, the antibody-protein complex could be observed for more than 7 cell cycles in vivo, revealing a dynamic redistribution from the nucleus to cytoplasm at each mitosis from blastoderm until hatching. The evaluation of two- and three-dimensional CLSM data sets demonstrated important differences in the localization of the protein in the nuclei of living compared to fixed embryos. The Hrb57A protein was recruited to the 93D locus upon heat shock and thus serves as an in vivo probe for the activity of the gene in diploid cells of the embryo. Observations during heat shock revealed considerable mobility within interphase nuclei of this transcription site. Furthermore, the reinitiation as well as the down regulation of transcriptional loci in vivo during the recovery from heat shock could be followed by the rapid redistribution of the hnRNP K during stress recovery. These data are incompatible with a model of the interphase nucleus in which transcription complexes are associated with a rigid nuclear matrix.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>9128243</pmid><doi>10.1083/jcb.137.2.291</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Antibodies Antibodies, Monoclonal Cell Nucleus - metabolism Cells Chromosomes Drosophila Drosophila melanogaster Drosophila melanogaster - embryology Embryo, Nonmammalian Embryos Gene Expression Regulation, Developmental Genes Genetic loci Heat-Shock Response - physiology Heterogeneous nuclear ribonucleoproteins Heterogeneous-Nuclear Ribonucleoprotein K Interphase Proteins Ribonucleoproteins - metabolism RNA RNA, Messenger - analysis Shock heating Transcription, Genetic - physiology Transcriptional Activation |
title | The Dynamic Nuclear Redistribution of an hnRNP K-Homologous Protein during Drosophila Embryo Development and Heat Shock. Flexibility of Transcription Sites in Vivo |
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