Crystal structure of thymidylate synthase A from Bacillus subtilis

• Published in: Protein science 1999-03, Vol.8 (3), p.538-544
• Main Authors: FOX, KRISTIN M., MALEY, FRANK, GARIBIAN, ARAIK, CHANGCHIEN, LI-MING, VAN ROEY, PATRICK
• Format: Article
• Language: English
• Subjects: Bacillus subtilis - enzymology; Bacillus subtilis thymidylate synthase; Base Sequence; Binding Sites; Cloning, Molecular; Dimerization; DNA Primers; Models, Molecular; structural comparison; ternary complex; Thymidylate Synthase - chemistry; Thymidylate Synthase - genetics; Thymidylate Synthase - metabolism
• ISSN: 0961-8368; 1469-896X
• DOI: 10.1110/ps.8.3.538

Thymidylate synthase (TS) converts dUMP to dTMP by reductive methylation, where 5,10-methylenetetrahydrofolate is the source of both the methylene group and reducing equivalents. The mechanism of this reaction has been extensively studied, mainly using the enzyme from Escherichia coli. Bacillus subtilis contains two genes for TSs, ThyA and ThyB. The ThyB enzyme is very similar to other bacterial TSs, but the ThyA enzyme is quite different, both in sequence and activity. In ThyA TS, the active site histidine is replaced by valine. In addition, the B. subtilis enzyme has a 2.4-fold greater kcat than the E. coli enzyme. The structure of B. subtilis thymidylate synthase in a ternary complex with 5-fluoro-dUMP and 5,10-methylenetetrahydrofolate has been determined to 2.5 Å resolution. Overall, the structure of B. subtilis TS (ThyA) is similar to that of the E. coli enzyme. However, there are significant differences in the structures of two loops, the dimer interface and the details of the active site. The effects of the replacement of histidine by valine and a serine to glutamine substitution in the active site area, and the addition of a loop over the carboxy terminus may account for the differences in kcat found between the two enzymes.