Resolution of ligand positions by site-directed tryptophan fluorescence in tear lipocalin

• Published in: Protein science 2000-02, Vol.9 (2), p.325-331
• Main Authors: GASYMOV, OKTAY K., ABDURAGIMOV, ADIL R., YUSIFOV, TALEH N., GLASGOW, BEN J.
• Format: Article
• Language: English
• Subjects: Apoproteins - chemistry; Binding Sites - genetics; Carrier Proteins - chemistry; Carrier Proteins - genetics; Circular Dichroism; Electron Spin Resonance Spectroscopy; Escherichia coli - genetics; Humans; In Vitro Techniques; ligand motion; Ligands; Lipocalin 1; Mutagenesis, Site-Directed; nitroxide quenching; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; site‐directed tryptophan fluorescence; Spectrometry, Fluorescence; tear lipocalin; Tears - chemistry; Tryptophan - chemistry; von Ebner's protein
• ISSN: 0961-8368; 1469-896X
• DOI: 10.1110/ps.9.2.325

The lipocalin superfamily of proteins functions in the binding and transport of a variety of important hydrophobic molecules. Tear lipocalin is a promiscuous lipid binding member of the family and serves as a paradigm to study the molecular determinants of ligand binding. Conserved regions in the lipocalins, such as the G strand and the F-G loop, may play an important role in ligand binding and delivery. We studied structural changes in the G strand of holo- and apo-tear lipocalin using spectroscopic methods including circular dichroism analysis and site-directed tryptophan fluorescence. Apo-tear lipocalin shows the same general structural characteristics as holo-tear lipocalin including alternating periodicity of a β-strand, orientation of amino acid residues 105, 103, 101, and 99 facing the cavity, and progressive depth in the cavity from residues 105 to 99. For amino acid residues facing the internal aspect of cavity, the presence of a ligand is associated with blue shifted spectra. The collisional rate constants indicate that these residues are not less exposed to solvent in holo-tear lipocalin than in apo-tear lipocalin. Rather the spectral blue shifts may be accounted for by a ligand induced rigidity in holo-TL. Amino acid residues 94 and 95 are consistent with positions in the F–G loop and show greater exposure to solvent in the holo- than the apo-proteins. These findings are consistent with the general hypothesis that the F–G loop in the holo-proteins of the lipocalin family is available for receptor interactions and delivery of ligands to specific targets. Site-directed tryptophan fluorescence was used in combination with a nitroxide spin labeled fatty acid analog to elucidate dynamic ligand interactions with specific amino acid residues. Collisional quenching constants of the nitroxide spin label provide evidence that at least three amino acids of the G strand residues interact with the ligand. Stern–Volmer plots are inconsistent with a ligand that is held in a static position in the calyx, but rather suggest that the ligand is in motion. The combination of site-directed tryptophan fluorescence with quenching by nitroxide labeled species has broad applicability in probing specific interactions in the solution structure of proteins and provides dynamic information that is not attainable by X-ray crystallography.