Relocation of Aurora B from Centromeres to the Central Spindle at the Metaphase to Anaphase Transition Requires MKlp2

Mitotic kinases of the Polo and Aurora families are key regulators of chromosome segregation and cytokinesis. Here, we have investigated the role of MKlp1 and MKlp2, two vertebrate mitotic kinesins essential for cytokinesis, in the spatial regulation of the Aurora B kinase. Previously, we have demon...

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Bibliographic Details
Published in:The Journal of cell biology 2004-07, Vol.166 (2), p.167-172
Main Authors: Gruneberg, Ulrike, Neef, Rüdiger, Honda, Reiko, Nigg, Erich A., Barr, Francis A.
Format: Article
Language:English
Subjects:
Anaphase
Antibodies
Aurora Kinase B
Aurora Kinases
Cell Cycle Proteins
Cells
Cellular biology
Centromere - metabolism
Centromeres
Chromosomal Proteins, Non-Histone - metabolism
Chromosome segregation
Cytokinesis
Enzymes
HeLa Cells
Humans
Kinesins - physiology
Macromolecular Substances
Metaphase
Microtubule-Associated Proteins - physiology
Phosphoric Monoester Hydrolases - metabolism
Phosphorylation
Polo-Like Kinase 1
Protein Kinases - metabolism
Protein Serine-Threonine Kinases - metabolism
Protein Transport
Protein Tyrosine Phosphatases
Proteins
Proto-Oncogene Proteins
Small interfering RNA
Spindle Apparatus - metabolism
Telophase
Zebrafish Proteins
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Summary:Mitotic kinases of the Polo and Aurora families are key regulators of chromosome segregation and cytokinesis. Here, we have investigated the role of MKlp1 and MKlp2, two vertebrate mitotic kinesins essential for cytokinesis, in the spatial regulation of the Aurora B kinase. Previously, we have demonstrated that MKlp2 recruits Polo-like kinase 1 (Plk1) to the central spindle in anaphase. We now find that in MKlp2 but not MKlp1-depleted cells the Aurora B-INCENP complex remains at the centromeres and fails to relocate to the central spindle. MKlp2 exerts dual control over Aurora B localization, because it is a binding partner for Aurora B, and furthermore for the phosphatase Cdc14A. Cdc14A can dephosphorylate INCENP and may contribute to its relocation to the central spindle in anaphase. We propose that MKlp2 is involved in the localization of Plk1, Aurora B, and Cdc14A to the central spindle during anaphase, and that the integration of signaling by these proteins is necessary for proper cytokinesis.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200403084