Pericentrin Forms a Complex with Intraflagellar Transport Proteins and Polycystin-2 and Is Required for Primary Cilia Assembly

Primary cilia are nonmotile microtubule structures that assemble from basal bodies by a process called intraflagellar transport (IFT) and are associated with several human diseases. Here, we show that the centrosome protein pericentrin (Pcnt) colocalizes with IFT proteins to the base of primary and...

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Bibliographic Details
Published in:The Journal of cell biology 2004-08, Vol.166 (5), p.637-643
Main Authors: Jurczyk, Agata, Gromley, Adam, Redick, Sambra, San Agustin, Jovenal, Witman, George, Pazour, Gregory J., Dorien J. M. Peters, Doxsey, Stephen
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigens - metabolism
Antigens - ultrastructure
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cell lines
Cell Movement - physiology
Cells
Cells, Cultured
Cellular biology
Centrioles
Centrosomes
Cilia
Cilia - metabolism
Cilia - ultrastructure
Disease
Drosophila
Epithelial cells
Epithelial Cells - metabolism
Epithelial Cells - ultrastructure
Immunohistochemistry
Lamins
Macromolecular Substances
Male
Membrane Proteins - metabolism
Membrane Proteins - ultrastructure
Mice
Microscopy, Electron
Protein Transport - physiology
Proteins
RNA Interference
Small interfering RNA
Spermatocytes - metabolism
Spermatocytes - ultrastructure
TRPP Cation Channels
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Summary:Primary cilia are nonmotile microtubule structures that assemble from basal bodies by a process called intraflagellar transport (IFT) and are associated with several human diseases. Here, we show that the centrosome protein pericentrin (Pcnt) colocalizes with IFT proteins to the base of primary and motile cilia. Immunogold electron microscopy demonstrates that Pcnt is on or near basal bodies at the base of cilia. Pcnt depletion by RNA interference disrupts basal body localization of IFT proteins and the cation channel polycystin-2 (PC2), and inhibits primary cilia assembly in human epithelial cells. Conversely, silencing of IFT20 mislocalizes Pcnt from basal bodies and inhibits primary cilia assembly. Pcnt is found in spermatocyte IFT fractions, and IFT proteins are found in isolated centrosome fractions. Pcnt antibodies coimmunoprecipitate IFT proteins and PC2 from several cell lines and tissues. We conclude that Pcnt, IFTs, and PC2 form a complex in vertebrate cells that is required for assembly of primary cilia and possibly motile cilia and flagella.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200405023