Neural Cell Adhesion Molecule Promotes Accumulation of TGN Organelles at Sites of Neuron-to-Neuron Contacts

Transformation of a contact between axon and dendrite into a synapse is accompanied by accumulation of the synaptic machinery at this site, being delivered in intracellular organelles mainly of TGN origin. Here, we report that in cultured hippocampal neurons, TGN organelles are linked via spectrin t...

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Bibliographic Details
Published in:The Journal of cell biology 2002-11, Vol.159 (4), p.649-661
Main Authors: Sytnyk, Vladimir, Iryna Leshchyns'ka, Delling, Markus, Dityateva, Galina, Dityatev, Alexander, Schachner, Melitta
Format: Article
Language:English
Subjects:
Animals
Antibodies
Axons
Biological Transport - physiology
Cell Membrane - metabolism
Cell membranes
Cells, Cultured
Cellular biology
Coatomer Protein - metabolism
Dendrites
Dyes
Fluorescent Dyes - metabolism
Hippocampus - cytology
Hippocampus - metabolism
Mice
Mice, Inbred C57BL
Microscopy, Confocal
Molecules
Neural cell adhesion molecules
Neural Cell Adhesion Molecules - genetics
Neural Cell Adhesion Molecules - metabolism
Neurites
Neurons
Neurons - cytology
Neurons - metabolism
Organelles
Organelles - metabolism
Pyridinium Compounds - metabolism
Quaternary Ammonium Compounds - metabolism
Recombinant Fusion Proteins - metabolism
Spectrin - metabolism
Synapses
Synapses - metabolism
Synaptophysin - metabolism
Time Factors
trans-Golgi Network - metabolism
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Summary:Transformation of a contact between axon and dendrite into a synapse is accompanied by accumulation of the synaptic machinery at this site, being delivered in intracellular organelles mainly of TGN origin. Here, we report that in cultured hippocampal neurons, TGN organelles are linked via spectrin to clusters of the neural cell adhesion molecule (NCAM) in the plasma membrane. These complexes are translocated along neurites and trapped at sites of initial neurite-to-neurite contacts within several minutes after initial contact formation. The accumulation of TGN organelles at contacts with NCAM-deficient neurons is reduced when compared with wild-type cells, suggesting that NCAM mediates the anchoring of intracellular organelles in nascent synapses.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200205098