In vivo Binding of Active Heat Shock Transcription Factor 1 to Human Chromosome 9 Heterochromatin during Stress

Activation of the mammalian heat shock transcription factor (HSF)1 by stress is a multistep process resulting in the transcription of heat shock genes. Coincident with these events is the rapid and reversible redistribution of HSF1 to discrete nuclear structures termed HSF1 granules, whose function...

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Bibliographic Details
Published in:The Journal of cell biology 2002-03, Vol.156 (5), p.775-781
Main Authors: Jolly, Caroline, Konecny, Lara, Grady, Deborah L., Kutskova, Yulia A., Cotto, José J., Morimoto, Richard I., Claire Vourc'h
Format: Article
Language:English
Subjects:
Antibodies
Artificial satellites
Binding Sites - genetics
Cell Nucleus - genetics
Cell Nucleus - metabolism
Cells, Cultured
Cellular biology
chromosome 9
Chromosomes
Chromosomes, Human, Pair 9 - genetics
Cytoplasmic Granules - genetics
Cytoplasmic Granules - metabolism
Deoxyribonucleic acid
DNA
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Female
Fluorescent Antibody Technique
Fluorescent antibody techniques
Genes
heat shock factor 1
Heat Shock Transcription Factors
Heat-Shock Proteins - genetics
Heat-Shock Proteins - metabolism
HeLa cells
Heterochromatin
Heterochromatin - genetics
HSF1 protein
Humans
Microsatellite Repeats - genetics
Protein Structure, Tertiary - genetics
Proteins
Shock heating
Space probes
Stress, Physiological - genetics
Stress, Physiological - metabolism
Transcription Factors
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Summary:Activation of the mammalian heat shock transcription factor (HSF)1 by stress is a multistep process resulting in the transcription of heat shock genes. Coincident with these events is the rapid and reversible redistribution of HSF1 to discrete nuclear structures termed HSF1 granules, whose function is still unknown. Key features are that the number of granules correlates with cell ploidy, suggesting the existence of a chromosomal target. Here we show that in humans, HSF1 granules localize to the 9q11-q12 heterochromatic region. Within this locus, HSF1 binds through direct DNA-protein interaction with a nucleosome-containing subclass of satellite III repeats. HSF1 granule formation only requires the DNA binding competence and the trimerization of the factor. This is the first example of a transcriptional activator that accumulates transiently and reversibly on a chromosome-specific heterochromatic locus.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200109018