Fluorescence Resonance Energy Transfer (FRET) Microscopy Imaging of Live Cell Protein Localizations

The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resoluti...

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Bibliographic Details
Published in:The Journal of cell biology 2003-03, Vol.160 (5), p.629-633
Main Authors: Sekar, Rajesh Babu, Periasamy, Ammasi
Format: Article
Language:English
Subjects:
Animals
Cells
Cellular biology
Emission spectra
Energy
Energy transfer
Eukaryotic Cells - cytology
Eukaryotic Cells - metabolism
Fluorescence
Fluorescence Resonance Energy Transfer - methods
Fluorescence Resonance Energy Transfer - trends
Fluorescent Dyes
Green Fluorescent Proteins
Humans
Image Processing, Computer-Assisted - methods
Image Processing, Computer-Assisted - trends
Imaging
Luminescent Proteins
Microscopy
Microscopy, Fluorescence - methods
Microscopy, Fluorescence - trends
Mini-Review
Mini-Reviews
Molecular imaging
NIH 3T3 cells
Proteins
Proteins - metabolism
Receptors
Scientific imaging
Spectroscopy
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Summary:The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200210140