A Direct Interaction of Axonin-1 with NgCAM-Related Cell Adhesion Molecule (NrCAM) Results in Guidance, but not Growth of Commissural Axons

An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elonga...

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Bibliographic Details
Published in:The Journal of cell biology 2000-05, Vol.149 (4), p.951-968
Main Authors: Fitzli, Dora, Stoeckli, Esther T., Kunz, Stefan, Siribour, Kingsley, Rader, Christoph, Kunz, Beat, Kozlov, Serguei V., Buchstaller, Andrea, Lane, Robert P., Suter, Daniel M., Dreyer, William J., Sonderegger, Peter
Format: Article
Language:English
Subjects:
Animals
Antibodies
Axons
Axons - physiology
Binding Sites
Cell adhesion
Cell Adhesion - physiology
Cell Adhesion Molecules
Cell Adhesion Molecules, Neuron-Glia - metabolism
Cell Adhesion Molecules, Neuronal - genetics
Cell Adhesion Molecules, Neuronal - metabolism
Cell aggregates
Cells, Cultured
Cellular biology
Chick Embryo
Contactin 2
Embryonic Induction
Growth cones
Growth Cones - physiology
Molecules
Multigene Family
Neural Pathways - embryology
Neurites
Neurons
Original
Physical growth
Protein Binding
Recombinant Proteins - metabolism
Spinal Cord - cytology
Spinal Cord - embryology
Spinal Cord - surgery
Stripes
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Summary:An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti-axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.149.4.951