Loading…
A Direct Interaction of Axonin-1 with NgCAM-Related Cell Adhesion Molecule (NrCAM) Results in Guidance, but not Growth of Commissural Axons
An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elonga...
Saved in:
| Published in: | The Journal of cell biology 2000-05, Vol.149 (4), p.951-968 |
|---|---|
| Main Authors: | , , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c3481-cdcacceeb22702f97a72c7067058510f1aa79042dd3d88b44362265eca56da323 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c3481-cdcacceeb22702f97a72c7067058510f1aa79042dd3d88b44362265eca56da323 |
| container_end_page | 968 |
| container_issue | 4 |
| container_start_page | 951 |
| container_title | The Journal of cell biology |
| container_volume | 149 |
| creator | Fitzli, Dora Stoeckli, Esther T. Kunz, Stefan Siribour, Kingsley Rader, Christoph Kunz, Beat Kozlov, Serguei V. Buchstaller, Andrea Lane, Robert P. Suter, Daniel M. Dreyer, William J. Sonderegger, Peter |
| description | An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti-axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth. |
| doi_str_mv | 10.1083/jcb.149.4.951 |
| format | article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2174557</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>1619854</jstor_id><sourcerecordid>1619854</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3481-cdcacceeb22702f97a72c7067058510f1aa79042dd3d88b44362265eca56da323</originalsourceid><addsrcrecordid>eNpdkUFv1DAQhS0EokvhyA0hiwMCiSwex46dC1IUYKnUFqmCs-U43q5XWbu1HQq_gT-Nl62gcJrDfPPmzTyEngJZApH1260ZlsDaJVu2HO6hBXBGKgmM3EcLQihULaf8CD1KaUsIYYLVD9FRmQSQNVugnx1-76I1GZ_4bKM22QWPwxp334N3vgJ84_IGn1_23Vl1YSed7Yh7O024Gzc27eGzMFkzTxa_Oo-Feo0vbJqnnLDzeDW7UXtj3-BhztiHjFcx3BTBsqEPu51LaY56-r0tPUYP1npK9sltPUZfP3740n-qTj-vTvrutDI1k1CZ0WhjrB0oFYSuW6EFNYI0gnDJgaxBa9ESRsexHqUcGKsbShtujebNqGtaH6N3B92redjZ0Vifiwd1Fd1Oxx8qaKf-7Xi3UZfhm6IgGOeiCLy8FYjherYpq3KIKU_R3oY5KQHAKTRQwBf_gdswR1-O22sRSRq5h6oDZGJIKdr1HydA1D5jVTJWJWPFVMm48M_v2r9DH0ItwLMDsE05xL_9BlrJWf0L0yCrdg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217080681</pqid></control><display><type>article</type><title>A Direct Interaction of Axonin-1 with NgCAM-Related Cell Adhesion Molecule (NrCAM) Results in Guidance, but not Growth of Commissural Axons</title><source>JSTOR Archival Journals and Primary Sources Collection</source><creator>Fitzli, Dora ; Stoeckli, Esther T. ; Kunz, Stefan ; Siribour, Kingsley ; Rader, Christoph ; Kunz, Beat ; Kozlov, Serguei V. ; Buchstaller, Andrea ; Lane, Robert P. ; Suter, Daniel M. ; Dreyer, William J. ; Sonderegger, Peter</creator><creatorcontrib>Fitzli, Dora ; Stoeckli, Esther T. ; Kunz, Stefan ; Siribour, Kingsley ; Rader, Christoph ; Kunz, Beat ; Kozlov, Serguei V. ; Buchstaller, Andrea ; Lane, Robert P. ; Suter, Daniel M. ; Dreyer, William J. ; Sonderegger, Peter</creatorcontrib><description>An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti-axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.149.4.951</identifier><identifier>PMID: 10811834</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Animals ; Antibodies ; Axons ; Axons - physiology ; Binding Sites ; Cell adhesion ; Cell Adhesion - physiology ; Cell Adhesion Molecules ; Cell Adhesion Molecules, Neuron-Glia - metabolism ; Cell Adhesion Molecules, Neuronal - genetics ; Cell Adhesion Molecules, Neuronal - metabolism ; Cell aggregates ; Cells, Cultured ; Cellular biology ; Chick Embryo ; Contactin 2 ; Embryonic Induction ; Growth cones ; Growth Cones - physiology ; Molecules ; Multigene Family ; Neural Pathways - embryology ; Neurites ; Neurons ; Original ; Physical growth ; Protein Binding ; Recombinant Proteins - metabolism ; Spinal Cord - cytology ; Spinal Cord - embryology ; Spinal Cord - surgery ; Stripes</subject><ispartof>The Journal of cell biology, 2000-05, Vol.149 (4), p.951-968</ispartof><rights>Copyright 2000 The Rockefeller University Press</rights><rights>Copyright Rockefeller University Press May 15, 2000</rights><rights>2000 The Rockefeller University Press 2000 The Rockefeller University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3481-cdcacceeb22702f97a72c7067058510f1aa79042dd3d88b44362265eca56da323</citedby><cites>FETCH-LOGICAL-c3481-cdcacceeb22702f97a72c7067058510f1aa79042dd3d88b44362265eca56da323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1619854$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1619854$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,881,27903,27904,58216,58449</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10811834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fitzli, Dora</creatorcontrib><creatorcontrib>Stoeckli, Esther T.</creatorcontrib><creatorcontrib>Kunz, Stefan</creatorcontrib><creatorcontrib>Siribour, Kingsley</creatorcontrib><creatorcontrib>Rader, Christoph</creatorcontrib><creatorcontrib>Kunz, Beat</creatorcontrib><creatorcontrib>Kozlov, Serguei V.</creatorcontrib><creatorcontrib>Buchstaller, Andrea</creatorcontrib><creatorcontrib>Lane, Robert P.</creatorcontrib><creatorcontrib>Suter, Daniel M.</creatorcontrib><creatorcontrib>Dreyer, William J.</creatorcontrib><creatorcontrib>Sonderegger, Peter</creatorcontrib><title>A Direct Interaction of Axonin-1 with NgCAM-Related Cell Adhesion Molecule (NrCAM) Results in Guidance, but not Growth of Commissural Axons</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti-axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Axons</subject><subject>Axons - physiology</subject><subject>Binding Sites</subject><subject>Cell adhesion</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Adhesion Molecules</subject><subject>Cell Adhesion Molecules, Neuron-Glia - metabolism</subject><subject>Cell Adhesion Molecules, Neuronal - genetics</subject><subject>Cell Adhesion Molecules, Neuronal - metabolism</subject><subject>Cell aggregates</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Chick Embryo</subject><subject>Contactin 2</subject><subject>Embryonic Induction</subject><subject>Growth cones</subject><subject>Growth Cones - physiology</subject><subject>Molecules</subject><subject>Multigene Family</subject><subject>Neural Pathways - embryology</subject><subject>Neurites</subject><subject>Neurons</subject><subject>Original</subject><subject>Physical growth</subject><subject>Protein Binding</subject><subject>Recombinant Proteins - metabolism</subject><subject>Spinal Cord - cytology</subject><subject>Spinal Cord - embryology</subject><subject>Spinal Cord - surgery</subject><subject>Stripes</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNpdkUFv1DAQhS0EokvhyA0hiwMCiSwex46dC1IUYKnUFqmCs-U43q5XWbu1HQq_gT-Nl62gcJrDfPPmzTyEngJZApH1260ZlsDaJVu2HO6hBXBGKgmM3EcLQihULaf8CD1KaUsIYYLVD9FRmQSQNVugnx1-76I1GZ_4bKM22QWPwxp334N3vgJ84_IGn1_23Vl1YSed7Yh7O024Gzc27eGzMFkzTxa_Oo-Feo0vbJqnnLDzeDW7UXtj3-BhztiHjFcx3BTBsqEPu51LaY56-r0tPUYP1npK9sltPUZfP3740n-qTj-vTvrutDI1k1CZ0WhjrB0oFYSuW6EFNYI0gnDJgaxBa9ESRsexHqUcGKsbShtujebNqGtaH6N3B92redjZ0Vifiwd1Fd1Oxx8qaKf-7Xi3UZfhm6IgGOeiCLy8FYjherYpq3KIKU_R3oY5KQHAKTRQwBf_gdswR1-O22sRSRq5h6oDZGJIKdr1HydA1D5jVTJWJWPFVMm48M_v2r9DH0ItwLMDsE05xL_9BlrJWf0L0yCrdg</recordid><startdate>20000515</startdate><enddate>20000515</enddate><creator>Fitzli, Dora</creator><creator>Stoeckli, Esther T.</creator><creator>Kunz, Stefan</creator><creator>Siribour, Kingsley</creator><creator>Rader, Christoph</creator><creator>Kunz, Beat</creator><creator>Kozlov, Serguei V.</creator><creator>Buchstaller, Andrea</creator><creator>Lane, Robert P.</creator><creator>Suter, Daniel M.</creator><creator>Dreyer, William J.</creator><creator>Sonderegger, Peter</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20000515</creationdate><title>A Direct Interaction of Axonin-1 with NgCAM-Related Cell Adhesion Molecule (NrCAM) Results in Guidance, but not Growth of Commissural Axons</title><author>Fitzli, Dora ; Stoeckli, Esther T. ; Kunz, Stefan ; Siribour, Kingsley ; Rader, Christoph ; Kunz, Beat ; Kozlov, Serguei V. ; Buchstaller, Andrea ; Lane, Robert P. ; Suter, Daniel M. ; Dreyer, William J. ; Sonderegger, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3481-cdcacceeb22702f97a72c7067058510f1aa79042dd3d88b44362265eca56da323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Axons</topic><topic>Axons - physiology</topic><topic>Binding Sites</topic><topic>Cell adhesion</topic><topic>Cell Adhesion - physiology</topic><topic>Cell Adhesion Molecules</topic><topic>Cell Adhesion Molecules, Neuron-Glia - metabolism</topic><topic>Cell Adhesion Molecules, Neuronal - genetics</topic><topic>Cell Adhesion Molecules, Neuronal - metabolism</topic><topic>Cell aggregates</topic><topic>Cells, Cultured</topic><topic>Cellular biology</topic><topic>Chick Embryo</topic><topic>Contactin 2</topic><topic>Embryonic Induction</topic><topic>Growth cones</topic><topic>Growth Cones - physiology</topic><topic>Molecules</topic><topic>Multigene Family</topic><topic>Neural Pathways - embryology</topic><topic>Neurites</topic><topic>Neurons</topic><topic>Original</topic><topic>Physical growth</topic><topic>Protein Binding</topic><topic>Recombinant Proteins - metabolism</topic><topic>Spinal Cord - cytology</topic><topic>Spinal Cord - embryology</topic><topic>Spinal Cord - surgery</topic><topic>Stripes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fitzli, Dora</creatorcontrib><creatorcontrib>Stoeckli, Esther T.</creatorcontrib><creatorcontrib>Kunz, Stefan</creatorcontrib><creatorcontrib>Siribour, Kingsley</creatorcontrib><creatorcontrib>Rader, Christoph</creatorcontrib><creatorcontrib>Kunz, Beat</creatorcontrib><creatorcontrib>Kozlov, Serguei V.</creatorcontrib><creatorcontrib>Buchstaller, Andrea</creatorcontrib><creatorcontrib>Lane, Robert P.</creatorcontrib><creatorcontrib>Suter, Daniel M.</creatorcontrib><creatorcontrib>Dreyer, William J.</creatorcontrib><creatorcontrib>Sonderegger, Peter</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fitzli, Dora</au><au>Stoeckli, Esther T.</au><au>Kunz, Stefan</au><au>Siribour, Kingsley</au><au>Rader, Christoph</au><au>Kunz, Beat</au><au>Kozlov, Serguei V.</au><au>Buchstaller, Andrea</au><au>Lane, Robert P.</au><au>Suter, Daniel M.</au><au>Dreyer, William J.</au><au>Sonderegger, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Direct Interaction of Axonin-1 with NgCAM-Related Cell Adhesion Molecule (NrCAM) Results in Guidance, but not Growth of Commissural Axons</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>2000-05-15</date><risdate>2000</risdate><volume>149</volume><issue>4</issue><spage>951</spage><epage>968</epage><pages>951-968</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti-axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>10811834</pmid><doi>10.1083/jcb.149.4.951</doi><tpages>18</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9525 |
| ispartof | The Journal of cell biology, 2000-05, Vol.149 (4), p.951-968 |
| issn | 0021-9525 1540-8140 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2174557 |
| source | JSTOR Archival Journals and Primary Sources Collection |
| subjects | Animals Antibodies Axons Axons - physiology Binding Sites Cell adhesion Cell Adhesion - physiology Cell Adhesion Molecules Cell Adhesion Molecules, Neuron-Glia - metabolism Cell Adhesion Molecules, Neuronal - genetics Cell Adhesion Molecules, Neuronal - metabolism Cell aggregates Cells, Cultured Cellular biology Chick Embryo Contactin 2 Embryonic Induction Growth cones Growth Cones - physiology Molecules Multigene Family Neural Pathways - embryology Neurites Neurons Original Physical growth Protein Binding Recombinant Proteins - metabolism Spinal Cord - cytology Spinal Cord - embryology Spinal Cord - surgery Stripes |
| title | A Direct Interaction of Axonin-1 with NgCAM-Related Cell Adhesion Molecule (NrCAM) Results in Guidance, but not Growth of Commissural Axons |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T21%3A28%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Direct%20Interaction%20of%20Axonin-1%20with%20NgCAM-Related%20Cell%20Adhesion%20Molecule%20(NrCAM)%20Results%20in%20Guidance,%20but%20not%20Growth%20of%20Commissural%20Axons&rft.jtitle=The%20Journal%20of%20cell%20biology&rft.au=Fitzli,%20Dora&rft.date=2000-05-15&rft.volume=149&rft.issue=4&rft.spage=951&rft.epage=968&rft.pages=951-968&rft.issn=0021-9525&rft.eissn=1540-8140&rft.coden=JCLBA3&rft_id=info:doi/10.1083/jcb.149.4.951&rft_dat=%3Cjstor_pubme%3E1619854%3C/jstor_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3481-cdcacceeb22702f97a72c7067058510f1aa79042dd3d88b44362265eca56da323%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=217080681&rft_id=info:pmid/10811834&rft_jstor_id=1619854&rfr_iscdi=true |