Pentatricopeptide Repeat Gene OTP43 Is Required for trans-Splicing of the Mitochondrial nad1 Intron 1 in Arabidopsis thaliana

The mitochondrial NADH:ubiquinone oxidoreductase complex (Complex I) is a large protein complex formed from both nuclearly and mitochondrially encoded subunits. Subunit ND1 is encoded by a mitochondrial gene comprising five exons, and the mature transcript requires four RNA splicing events, two of w...

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Bibliographic Details
Published in:The Plant cell 2007-10, Vol.19 (10), p.3256-3265
Main Authors: de Longevialle, Andéol Falcon, Meyer, Etienne H, Andrés, Charles, Taylor, Nicolas L, Lurin, Claire, Millar, A. Harvey, Small, Ian D
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Base Pairing - genetics
Chloroplasts
DNA, Bacterial - genetics
Electron Transport Complex I - genetics
Electron Transport Complex I - metabolism
Electrophoresis, Polyacrylamide Gel
Exons - genetics
Gels
Gene Expression Regulation, Plant
Genes
Genetic Complementation Test
Immunoblotting
Introns
Mass Spectrometry
Mitochondria
Mitochondrial Proteins - genetics
Mitochondrial Proteins - metabolism
Models, Genetic
Mutation
Phenotype
Plant cells
Plant growth
Plants
Reverse Transcriptase Polymerase Chain Reaction
RNA
RNA Editing - genetics
RNA Splicing
Splicing
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Summary:The mitochondrial NADH:ubiquinone oxidoreductase complex (Complex I) is a large protein complex formed from both nuclearly and mitochondrially encoded subunits. Subunit ND1 is encoded by a mitochondrial gene comprising five exons, and the mature transcript requires four RNA splicing events, two of which involve trans-splicing independently transcribed RNAs. We have identified a nuclear gene (OTP43) absolutely required for trans-splicing of intron 1 (and only intron 1) of Arabidopsis thaliana nad1 transcripts. This gene encodes a previously uncharacterized pentatricopeptide repeat protein. Mutant Arabidopsis plants with a disrupted OTP43 gene do not present detectable mitochondrial Complex I activity and show severe defects in seed development, germination, and to a lesser extent in plant growth. The alternative respiratory pathway involving alternative oxidase is significantly induced in the mutant.
ISSN:1040-4651
1532-298X
1532-298X
DOI:10.1105/tpc.107.054841