CENP-Meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila

CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chr...

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Bibliographic Details
Published in:The Journal of cell biology 2000-07, Vol.150 (1), p.1-11
Main Authors: Yucel, Jennifer K., Marszalek, Janet D., McIntosh, J. Richard, Lawrence S. B. Goldstein, Cleveland, Don W., Philp, Alastair Valentine
Format: Article
Language:English
Subjects:
Anaphase
Animals
Antibodies
Cell cycle
Cell Cycle - genetics
Cell division
Cells
Cells, Cultured
CENP-meta
Centromere - metabolism
centromeres
Chromosomal Proteins, Non-Histone - genetics
Chromosome Segregation - genetics
Chromosomes
Cloning, Molecular
Drosophila
Drosophila Proteins
Embryos
Female
Gene Deletion
Genes
Genes, Lethal
Germ-Line Mutation
Kinetochores
Kinetochores - metabolism
Metaphase
Metaphase - genetics
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Mitosis
Mitosis - genetics
Molecular Motor Proteins - genetics
Molecular Motor Proteins - metabolism
Molecular Sequence Data
motor proteins
Mutagenesis, Insertional
Original
Proteins
Sequence Homology, Amino Acid
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Summary:CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of anaphase A, the CENP-meta protein remains associated with the centromeric/kinetochore region of the chromosome during all stages of the Drosophila cell cycle. P-element-mediated disruption of the CENP-meta gene leads to late larval/pupal stage lethality with incomplete chromosome alignment at metaphase. Complete removal of CENP-meta from the female germline leads to lethality in early embryos resulting from defects in metaphase chromosome alignment. Real-time imaging of these mutants with GFP-labeled chromosomes demonstrates that CENP-meta is required for the maintenance of chromosomes at the metaphase plate, demonstrating that the functions required to establish and maintain chromosome congression have distinguishable requirements.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.150.1.1