Role of hemolysin for the intracellular growth of Listeria monocytogenes

Listeria monocytogenes insertion mutants defective in hemolysin production were generated using the conjugative transposons Tn916 and Tn1545. All of the nonhemolytic mutants (hly-) lacked a secreted 58-kD polypeptide, presumedly hemolysin, and were avirulent in a mouse model. An intracellular multip...

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Bibliographic Details
Published in:The Journal of experimental medicine 1988-04, Vol.167 (4), p.1459-1471
Main Authors: PORTNOY, D. A, JACKS, P. S, HINRICHS, D. J
Format: Article
Language:English
Subjects:
Animals
Bacterial diseases
Bacterial Proteins - genetics
Bacterial Proteins - physiology
Biological and medical sciences
Cell Line
DNA Transposable Elements
Epithelial Cells
Epithelium - microbiology
Experimental bacterial diseases and models
Fibroblasts - microbiology
Hemolysin Proteins - genetics
Hemolysin Proteins - physiology
Humans
Infectious diseases
Listeria monocytogenes
Listeria monocytogenes - cytology
Listeria monocytogenes - genetics
Macrophages - microbiology
Medical sciences
Mice
Organ Specificity
Species Specificity
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Summary:Listeria monocytogenes insertion mutants defective in hemolysin production were generated using the conjugative transposons Tn916 and Tn1545. All of the nonhemolytic mutants (hly-) lacked a secreted 58-kD polypeptide, presumedly hemolysin, and were avirulent in a mouse model. An intracellular multiplication assay was established in monolayers of mouse bone marrow-derived macrophages, the J774 macrophage-like cell line, the CL.7 embryonic mouse fibroblast cell line, and the Henle 407 human epithelial cell line. The hly+ strain grew intracellularly in all of the tissue culture cells with a doubling time of approximately 60 min. In contrast, the hly- mutants failed to grow in the murine-derived tissue culture cells, but retained the ability to grow in the human tissue culture cells examined. Hemolytic-positive revertants were selected after passage of the hly- mutants through monolayers of J774 cells. In each case, the hemolytic revertants possessed the 58-kD polypeptide, were capable of intracellular growth in tissue culture monolayers and were virulent for mice.
ISSN:0022-1007
1540-9538
DOI:10.1084/jem.167.4.1459