A Protein Interaction Map for Cell Polarity Development

• Published in: The Journal of cell biology 2001-08, Vol.154 (3), p.549-571
• Main Authors: Drees, Becky L., Sundin, Bryan, Brazeau, Elizabeth, Caviston, Juliane P., Chen, Guang-Chao, Guo, Wei, Kozminski, Keith G., Lau, Michelle W., Moskow, John J., Tong, Amy, Schenkman, Laura R., McKenzie, Amos, Brennwald, Patrick, Longtine, Mark, Bi, Erfei, Chan, Clarence, Novick, Peter, Boone, Charles, Pringle, John R., Davis, Trisha N., Field, Stanley, Drubin, David G.
• Format: Article
• Language: English
• Subjects: Actins; Actins - metabolism; Bacterial Proteins - genetics; Bipolar disorder; cdc42 GTP-Binding Protein, Saccharomyces cerevisiae - genetics; cdc42 GTP-Binding Protein, Saccharomyces cerevisiae - metabolism; Cell growth; Cell Polarity - physiology; Cell walls; Cells; Cellular biology; Cytokines; Cytokinesis; Endocytosis - physiology; Exocytosis; Fungal Proteins - genetics; Fungal Proteins - metabolism; Genes, cdc - physiology; Luminescent Proteins - genetics; Mathematical functions; Microfilaments; Proteins; rho GTP-Binding Proteins - metabolism; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins; Secretory Vesicles - metabolism; Two-Hybrid System Techniques; Yeast; Yeasts
• ISSN: 0021-9525; 1540-8140
• DOI: 10.1083/jcb.200104057

Many genes required for cell polarity development in budding yeast have been identified and arranged into a functional hierarchy. Core elements of the hierarchy are widely conserved, underlying cell polarity development in diverse eukaryotes. To enumerate more fully the protein-protein interactions that mediate cell polarity development, and to uncover novel mechanisms that coordinate the numerous events involved, we carried out a large-scale two-hybrid experiment. 68 Gal4 DNA binding domain fusions of yeast proteins associated with the actin cytoskeleton, septins, the secretory apparatus, and Rho-type GTPase were used to screen an array of yeast transformants that express ∼90% of the predicted Saccharomyces cerevisiae open reading frames as Gal4 activation domain fusions. 191 protein-protein interactions were detected, of which 128 had not been described previously. 44 interactions implicated 20 previously uncharacterized proteins in cell polarity development. Further insights into possible roles of 13 of these proteins were revealed by their multiple two-hybrid interactions and by subcellular localization. Included in the interaction network were associations of Cdc42 and Rho1 pathways with proteins involved in exocytosis, septin organization, actin assembly, microtubule organization, autophagy, cytokinesis, and cell wall synthesis. Other interactions suggested direct connections between Rho1- and Cdc42-regulated pathways; the secretory apparatus and regulators of polarity establishment; actin assembly and the morphogenesis checkpoint; and the exocytic and endocytic machinery. In total, a network of interactions that provide an integrated response of signaling proteins, the cytoskeleton, and organelles to the spatial cues that direct polarity development was revealed.