Peri-Golgi Vesicles Contain Retrograde but not Anterograde Proteins Consistent with the Cisternal Progression Model of Intra-Golgi Transport

A cisternal progression mode of intra-Golgi transport requires that Golgi resident proteins recycle by peri-Golgi vesicles, whereas the alternative model of vesicular transport predicts anterograde cargo proteins to be present in such vesicles. We have used quantitative immuno-EM on NRK cells to dis...

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Bibliographic Details
Published in:The Journal of cell biology 2001-12, Vol.155 (7), p.1213-1224
Main Authors: Martínez-Menárguez, José A., Prekeris, Rytis, Viola M. J. Oorschot, Scheller, Richard, Slot, Jan W., Geuze, Hans J., Klumperman, Judith
Format: Article
Language:English
Subjects:
Animals
Antibodies
Autoantigens - metabolism
Cell biology
Cell Cycle - physiology
Cell Line
Cells
Cellular biology
Coat Protein Complex I
COP coated vesicles
Enzymes
Freight
Freight transport
Golgi apparatus
Golgi Apparatus - physiology
Golgi Apparatus - ultrastructure
Green Fluorescent Proteins
Intracellular Membranes - metabolism
Intracellular Membranes - ultrastructure
Kidney
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Membrane Glycoproteins
Membrane Proteins - metabolism
Microscopy, Immunoelectron
P branes
Protein Transport
Proteins
Rats
Receptors, Peptide - metabolism
Recombinant Proteins - metabolism
Recycling
Transfection
Vesicular stomatitis Indiana virus - physiology
Viral Envelope Proteins - metabolism
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Summary:A cisternal progression mode of intra-Golgi transport requires that Golgi resident proteins recycle by peri-Golgi vesicles, whereas the alternative model of vesicular transport predicts anterograde cargo proteins to be present in such vesicles. We have used quantitative immuno-EM on NRK cells to distinguish peri-Golgi vesicles from other vesicles in the Golgi region. We found significant levels of the Golgi resident enzyme mannosidase II and the transport machinery proteins giantin, KDEL-receptor, and rBet1 in coatomer protein I-coated cisternal rims and peri-Golgi vesicles. By contrast, when cells expressed vesicular stomatitis virus protein G this anterograde marker was largely absent from the peri-Golgi vesicles. These data suggest a role of peri-Golgi vesicles in recycling of Golgi residents, rather than an important role in anterograde transport.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200108029