Macrophage Podosomes Assemble at the Leading Lamella by Growth and Fragmentation

Podosomes are actin- and fimbrin-containing adhesions at the leading edge of macrophages. In cells transfected with β-actin-ECFP and L-fimbrin-EYFP, quantitative four-dimensional microscopy of podosome assembly shows that new adhesions arise at the cell periphery by one of two mechanisms; de novo po...

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Bibliographic Details
Published in:The Journal of cell biology 2003-05, Vol.161 (4), p.697-705
Main Authors: Evans, James G., Correia, Ivan, Krasavina, Olga, Watson, Nicki, Matsudaira, Paul
Format: Article
Language:English
Subjects:
Actins
Actins - metabolism
Cell Adhesion
Cell Line
Cell Movement
Cell Polarity
Cell Size
Cells
Fibrin - metabolism
Fibroblasts
Fluorescence
Focal adhesions
Humans
Integrins
Macrophages
Macrophages - cytology
Macrophages - metabolism
Microscopy
Microscopy, Confocal
Microscopy, Electron, Scanning
Microtubules
Microtubules - metabolism
Models, Biological
Physical growth
podosomes
Pseudopodia - chemistry
Pseudopodia - metabolism
Time Factors
Wiskott Aldrich syndrome
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Summary:Podosomes are actin- and fimbrin-containing adhesions at the leading edge of macrophages. In cells transfected with β-actin-ECFP and L-fimbrin-EYFP, quantitative four-dimensional microscopy of podosome assembly shows that new adhesions arise at the cell periphery by one of two mechanisms; de novo podosome assembly, or fission of a precursor podosome into daughter podosomes. The large podosome cluster precursor also appears to be an adhesion structure; it contains actin, fimbrin, integrin, and is in close apposition to the substratum. Microtubule inhibitors paclitaxel and demecolcine inhibit the turnover and polarized formation of podosomes, but not the turnover rate of actin in these structures. Because daughter podosomes and podosome cluster precursors are preferentially located at the leading edge, they may play a critical role in continually generating new sites of cell adhesion.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200212037