Telomerase RNA Localized in the Replication Band and Spherical Subnuclear Organelles in Hypotrichous Ciliates

The intranuclear distribution of telomere DNA-binding protein and telomerase RNA in hypotrichous ciliates was revealed by indirect fluorescent antibody staining and in situ hybridization. The Oxytricha telomere protein colocalized with DNA, both being dispersed throughout the macronucleus except for...

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Bibliographic Details
Published in:The Journal of cell biology 1995-07, Vol.130 (2), p.243-253
Main Authors: Fang, Guowei, Cech, Thomas R.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Base Sequence
Biochemistry
Cell Nucleus - chemistry
Cell Nucleus - enzymology
Cellular biology
Chromosomes
Deoxyribonucleic acid
DNA
DNA Nucleotidylexotransferase - analysis
DNA Replication
DNA, Protozoan - analysis
DNA-Binding Proteins - analysis
Euplotes - chemistry
Euplotes - cytology
Euplotes - enzymology
Fluorescent Antibody Technique
In situ hybridization
In Situ Hybridization, Fluorescence
Interphase
Laboratory staining techniques
Macronucleus
Molecular Sequence Data
Oxytricha - chemistry
Oxytricha - cytology
Oxytricha - enzymology
RNA
RNA, Protozoan - analysis
S Phase
Telomeres
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Summary:The intranuclear distribution of telomere DNA-binding protein and telomerase RNA in hypotrichous ciliates was revealed by indirect fluorescent antibody staining and in situ hybridization. The Oxytricha telomere protein colocalized with DNA, both being dispersed throughout the macronucleus except for numerous spherical foci that contained neither DNA nor the protein. Surprisingly, the telomerase RNA was concentrated in these foci; therefore, much of telomerase does not colocalize with telomeres. These foci persist through the cell cycle. They may represent sites of assembly, transport or stockpiling of telomerase and other ribonucleoproteins. During S phase, the macronuclear DNA replication machinery is organized into a disc-shaped structure called the replication band. Telomerase RNA is enriched in the replication band as judged by fluorescence intensity. We conclude that the localization of a subfraction of telomerase is coordinated with semiconservative DNA replication.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.130.2.243