Hormonal Regulation of Caveolae Internalization

Caveolae undergo a cyclic transition from a flat segment of membrane to a vesicle that then returns to the cell surface. Here we present evidence that this cycle depends on a population of protein kinase C-α (PKC-α) molecules that reside in the caveolae membrane where they phosphorylate a 90-kD prot...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 1995-11, Vol.131 (4), p.929-938
Main Authors: Smart, Eric J., Ying, Yun-shu, Richard G. W. Anderson
Format: Article
Language:English
Subjects:
Aluminum Compounds - pharmacology
Animals
Caveolae
Caveolins
Cell Line - physiology
Cell Line - ultrastructure
Cell Membrane - enzymology
Cell Membrane - physiology
Cell Membrane - ultrastructure
Cell membranes
Cells
Fluorides - pharmacology
Folic Acid - metabolism
Haplorhini
Histamine - physiology
Histamines
Hormones - physiology
Internalization
Isoenzymes - metabolism
Isoenzymes - pharmacology
Kidney - cytology
Membrane Proteins - physiology
P branes
Phorbol 12,13-Dibutyrate - pharmacology
Phosphatases
Phosphorylation
Physiological regulation
Protein Kinase C - metabolism
Protein Kinase C - pharmacology
Protein Kinase C-alpha
Receptors
Receptors, Growth Factor - metabolism
Signal Transduction - physiology
Subcellular Fractions
Substrate Specificity
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Caveolae undergo a cyclic transition from a flat segment of membrane to a vesicle that then returns to the cell surface. Here we present evidence that this cycle depends on a population of protein kinase C-α (PKC-α) molecules that reside in the caveolae membrane where they phosphorylate a 90-kD protein. This cycle can be interrupted by treatment of the cells with phorbol-12,13-dibutyrate or agents that raise the concentration of diacylglycerol in the cell. Each of these conditions displaces PKC-α from caveolae, inhibits the phosphorylation of the 90-kD protein, and prevents internalization. Caveolae also contain a protein phosphatase that dephosphorylates the 90-kD once PKC-α is gone. A similar dissociation of PKC-α from caveolae and inhibition of invagination was observed when cells were treated with histamine. This effect was blocked by pyrilamine but not cimetidine, indicating the involvement of histamine H1 receptors. These findings suggest that the caveolae internalization cycle is hormonally regulated.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.131.4.929