Binding between the Neural Cell Adhesion Molecules Axonin-1 and Nr-CAM/Bravo Is Involved in Neuron-Glia Interaction

Neural cell adhesion molecules of the immunoglobulin superfamily mediate cellular interactions via homophilic binding to identical molecules and heterophilic binding to other family members or structurally unrelated cell-surface glycoproteins. Here we report on an interaction between axonin-1 and Nr...

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Bibliographic Details
Published in:The Journal of cell biology 1995-11, Vol.131 (4), p.1067-1081
Main Authors: Suter, Daniel M., Pollerberg, G. Elisabeth, Buchstaller, Andrea, Giger, Roman J., Dreyer, William J., Sonderegger, Peter
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibody Specificity
Axons - physiology
Base Sequence
Cell Adhesion - physiology
Cell Adhesion Molecules
Cell Adhesion Molecules, Neuronal - immunology
Cell Adhesion Molecules, Neuronal - metabolism
Cell aggregates
Cells
Chick Embryo
Contactin 2
Cultured cells
DNA Primers - chemistry
Ganglia, Spinal - cytology
Ganglia, Spinal - embryology
Integrins - physiology
Microspheres
Molecular Sequence Data
Molecules
Neurites
Neurites - ultrastructure
Neuroglia
Neuroglia - cytology
Neuroglia - ultrastructure
Neuron glia cell adhesion molecules
Neurons
Neurons - cytology
Neurons - ultrastructure
Protein Binding - physiology
Schwann cells
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Description
Summary:Neural cell adhesion molecules of the immunoglobulin superfamily mediate cellular interactions via homophilic binding to identical molecules and heterophilic binding to other family members or structurally unrelated cell-surface glycoproteins. Here we report on an interaction between axonin-1 and Nr-CAM/Bravo. In search for novel ligands of axonin-1, fluorescent polystyrene microspheres conjugated with axonin-1 were found to bind to peripheral glial cells from dorsal root ganglia. By antibody blockage experiments an axonin-1 receptor on the glial cells was identified as Nr-CAM. The specificity of the interaction was confirmed with binding studies using purified axonin-1 and Nr-CAM. In cultures of dissociated dorsal root ganglia antibodies against axonin-1 and Nr-CAM perturbed the formation of contacts between neurites and peripheral glial cells. Together, these results implicate a binding between axonin-1 of the neuritic and Nr-CAM of the glial cell membrane in the early phase of axon ensheathment in the peripheral nervous system.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.131.4.1067