Development of FRET-based assays in the far-red using CdTe quantum dots

Colloidal quantum dots (QDs) are now commercially available in a biofunctionalized form, and Förster resonance energy transfer (FRET) between bioconjugated dots and fluorophores within the visible range has been observed. We are particularly interested in the far-red region, as from a biological per...

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Bibliographic Details
Published in:Journal of biomedicine & biotechnology 2007, Vol.2007 (7), p.54169-54169
Main Authors: Chong, E Z, Matthews, D R, Summers, H D, Njoh, K L, Errington, R J, Smith, P J
Format: Article
Language:English
Subjects:
Assaying
Chemical properties
Energy transformation
Nanotechnology
Semiconductors
Tellurium
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Summary:Colloidal quantum dots (QDs) are now commercially available in a biofunctionalized form, and Förster resonance energy transfer (FRET) between bioconjugated dots and fluorophores within the visible range has been observed. We are particularly interested in the far-red region, as from a biological perspective there are benefits in pushing to approximately 700 nm to minimize optical absorption (ABS) within tissue and to avoid cell autofluorescence. We report on FRET between streptavidin- (STV-) conjugated CdTe quantum dots, Qdot705-STV, with biotinylated DY731-Bio fluorophores in a donor-acceptor assay. We also highlight the changes in DY731-Bio absorptivity during the streptavidin-biotin binding process which can be attributed to the structural reorientation. For fluorescence beyond 700 nm, different alloy compositions are required for the QD core and these changes directly affect the fluorescence decay dynamics producing a marked biexponential decay with a long-lifetime component in excess of 100 nanoseconds. We compare the influence of the two QD relaxation routes upon FRET dynamics in the presence of DY731-Bio.
ISSN:1110-7243
1110-7251
DOI:10.1155/2007/54169