Identification in the mould Hypocrea jecorina of a gene encoding an NADP⁺: d-xylose dehydrogenase

A gene coding for an NADP⁺-dependent d-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with d-xylos...

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Bibliographic Details
Published in:FEMS microbiology letters 2007-12, Vol.277 (2), p.249-253
Main Authors: Berghäll, Suvi, Hilditch, Satu, Penttilä, Merja, Richard, Peter
Format: Article
Language:English
Subjects:
Aldehyde Reductase - genetics
Aldehyde Reductase - metabolism
Biological and medical sciences
d-xylose dehydrogenase
d-xylose metabolism
Dehydrogenase
Dehydrogenases
DNA, Complementary - isolation & purification
EC 1.1.1.175
Enzymes
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
Fungal Proteins - metabolism
Fungi
Gene Expression
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Histidine
Hypocrea - enzymology
Hypocrea - genetics
Hypocrea jecorina
Intermediates
Kinetics
Microbiology
Molecular Sequence Data
Mycology
NADP
NADP-linked d-xylose dehydrogenase
Recombinant Proteins - genetics
Recombinant Proteins - isolation & purification
Recombinant Proteins - metabolism
Research Letter
Saccharomyces cerevisiae - genetics
Substrate Specificity
Sugar
Trichoderma reesei
Xylitol
Xylose
Xylose - metabolism
Xylulose
Yeast
Yeasts
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Summary:A gene coding for an NADP⁺-dependent d-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with d-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP⁺. The Km values for d-xylose and NADP⁺ are 43 mM and 250 μM, respectively. The role of this enzyme in H. jecorina is unclear because in this organism d-xylose is predominantly catabolized through a path with xylitol and d-xylulose as intermediates and the mould is unable to grow on d-xylonic acid.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2007.00969.x