Local expression of CYP19A1 and CYP19A2 in developing and adult killifish ( Fundulus heteroclitus )

Abstract P450 aromatase (CYP19) is the terminal enzyme in the steroidogenic pathway and catalyzes the conversion of androgens to estrogens. Fundulus heteroclitus like other teleosts, express two CYP19 genes, CYP19A1 and CYP19A2. The expression of CYP19s in Fundulus was measured by in situ hybridizat...

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Bibliographic Details
Published in:General and comparative endocrinology 2008-01, Vol.155 (2), p.307-317
Main Authors: Dong, Wu, Willett, Kristine L
Format: Article
Language:English
Subjects:
Animals
Aromatase
Aromatase - metabolism
Brain
CYP19
Endocrinology & Metabolism
Female
Fish Proteins - metabolism
Fundulidae - embryology
Fundulidae - metabolism
Fundulus
Fundulus heteroclitus
Gene Expression Regulation, Developmental - physiology
Isoenzymes
Male
Marine
Neurons - cytology
Neurons - enzymology
Oocyte
Oocytes - enzymology
Ovary - embryology
Ovary - enzymology
Teleostei
Testis - embryology
Testis - enzymology
Tissue Distribution
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Summary:Abstract P450 aromatase (CYP19) is the terminal enzyme in the steroidogenic pathway and catalyzes the conversion of androgens to estrogens. Fundulus heteroclitus like other teleosts, express two CYP19 genes, CYP19A1 and CYP19A2. The expression of CYP19s in Fundulus was measured by in situ hybridization throughout development. In 90 dpf (day post-fertilization) fish and adult fish, CYP19A1 was expressed in the ooplasm of early stage I oocytes (primary growth stage). Expression of CYP19A1 was localized in the follicle cell layer of late stage I (previtellogenic stage) and stage II (vitellogenic stage) follicles, but by stage III (early maturational follicles) CYP19A1 expression was localized in the vitelline envelope. Overall, CYP19A1 oocyte membrane expression gradually declined from highest expression at late stage I to nondetectable levels by stage IV. Highest expression of CYP19A2 was detected in the brain including the hypothalamus from 4, 6, 8, 10, 14 dpf embryos, 90 dpf fry fish and adult fish brain. In females compared to males, there was higher CYP19A2 expression in olfactory bulb. In addition to the brain, there was strong CYP19A2 signal in adrenal/kidney cells in 6–14 dpf embryos. This work establishes the localization and constitutive expression of CYP19s in Fundulus which can then be compared with potential disruption of CYP19A1 and CYP19A2 expression and physiological consequences caused by environmental contaminants.
ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2007.05.018