Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate

Phosphoinositides play a central role in regulating processes at intracellular membranes. In yeast, a large number of phospholipid biosynthetic enzymes use a common mechanism for transcriptional regulation. Yet, how the expression of genes encoding lipid kinases and phosphatases is regulated remains...

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Bibliographic Details
Published in:BMC molecular biology 2008-01, Vol.9 (1), p.16-16, Article 16
Main Authors: Knödler, Andreas, Konrad, Gerlinde, Mayinger, Peter
Format: Article
Language:English
Subjects:
5' Untranslated Regions - genetics
5' Untranslated Regions - metabolism
Cell membranes
Gene expression
Gene Expression Regulation, Enzymologic - physiology
Gene Expression Regulation, Fungal - physiology
Genetic aspects
Health aspects
Methods
Phosphatidylinositol
Phosphatidylinositol 4,5-Diphosphate - genetics
Phosphatidylinositol 4,5-Diphosphate - metabolism
Phosphoric Monoester Hydrolases - biosynthesis
Phosphoric Monoester Hydrolases - genetics
Physiological aspects
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - biosynthesis
Saccharomyces cerevisiae Proteins - genetics
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Summary:Phosphoinositides play a central role in regulating processes at intracellular membranes. In yeast, a large number of phospholipid biosynthetic enzymes use a common mechanism for transcriptional regulation. Yet, how the expression of genes encoding lipid kinases and phosphatases is regulated remains unknown. Here we show that the expression of lipid phosphatase Sac1p in the yeast Saccharomyces cerevisiae is regulated in response to changes in phosphatidylinositol-4-phosphate (PI(4)P) concentrations. Unlike genes encoding enzymes involved in phospholipid biosynthesis, expression of the SAC1 gene is independent of inositol levels. We identified a novel 9-bp motif within the 5' untranslated region (5'-UTR) of SAC1 that is responsible for PI(4)P-mediated regulation. Upregulation of SAC1 promoter activity correlates with elevated levels of Sac1 protein levels. Regulation of Sac1p expression via the concentration of its major substrate PI(4)P ensures proper maintenance of compartment-specific pools of PI(4)P.
ISSN:1471-2199
1471-2199
DOI:10.1186/1471-2199-9-16