Determination of Glycosyltransferase Specificities for the Escherichia coli O111 O Antigen by a Generic Approach

We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of the Escherichia coli O111 O-ant...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2008-02, Vol.74 (4), p.1294-1298
Main Authors: Stevenson, Gordon, Dieckelmann, Manuela, Reeves, Peter R
Format: Article
Language:English
Subjects:
Antigens
Bacteriology
Biochemistry
Biological and medical sciences
Cloning, Molecular
E coli
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genetics and Molecular Biology
Glucosyltransferases - genetics
Glucosyltransferases - metabolism
Microbiology
Models, Biological
Multigene Family
O Antigens - genetics
O Antigens - metabolism
Oligonucleotides - genetics
Species Specificity
Substrate Specificity
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Description
Summary:We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of the Escherichia coli O111 O-antigen GT genes.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.02660-07