Both pituitary and placental growth hormone transcripts are expressed in human peripheral blood mononuclear cells (PBMC)

Summary The hGH‐V gene codes for a variant of human pituitary growth hormone (hGH‐N) named placental growth hormone (hPGH). hPGH shares 93% amino acid identity with hGH‐N. Until now the hGH‐V gene was considered to be exclusively expressed in human placenta, where it replaces maternal circulating hG...

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Bibliographic Details
Published in:Clinical and experimental immunology 1997-11, Vol.110 (2), p.336-340
Main Authors: MELEN, L., HENNEN, G., DULLAART, R. P. F., HEINEN, E., IGOUT, A.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Female
Fundamental and applied biological sciences. Psychology
Gene Expression
Growth Hormone - biosynthesis
Growth Hormone - genetics
hGH-V
Human Growth Hormone - biosynthesis
Human Growth Hormone - genetics
Human health sciences
human pituitary growth hormone
Humans
Immunologie & maladie infectieuse
Immunology & infectious disease
Leukocytes, Mononuclear - metabolism
Male
Molecular and cellular biology
Molecular genetics
Original
peripheral blood monnuclear cells
peripheral blood mononuclear cells
Placental Hormones - biosynthesis
Placental Hormones - genetics
Polymerase Chain Reaction
Pregnancy
RNA, Messenger - analysis
RNA, Messenger - genetics
Sciences de la santé humaine
Transcription, Genetic
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Summary:Summary The hGH‐V gene codes for a variant of human pituitary growth hormone (hGH‐N) named placental growth hormone (hPGH). hPGH shares 93% amino acid identity with hGH‐N. Until now the hGH‐V gene was considered to be exclusively expressed in human placenta, where it replaces maternal circulating hGH‐N at the end of pregnancy. In this study we investigated by reverse transcriptase‐polymerase chain reaction (RT‐PCR) analysis hGH‐N, and hGH‐V, gene expression in PBMC in men, women and pregnant women. We have demonstrated that hGH‐N and hGH‐V transcripts are simultaneously produced by PBMC in both men and women as well as pregnant women. The PBMC of a PIT‐.1‐negative woman expressed only the hGH‐V transcript, but not the hGH‐N one as expected. In conclusion, hGH‐V mRNA is expressed by cells other than the syncytiotrophoblast, is not regulated by PIT‐1, and may be involved in immune regulation, as is pituitary GH.
ISSN:0009-9104
1365-2249
1365-2249
DOI:10.1111/j.1365-2249.1997.tb08337.x